MORPHOLOGICAL-STUDIES OF THE MICROCIRCULATORY SYSTEM OF PERIOVULATORY OVINE FOLLICLES
MORPHOLOGICAL-STUDIES OF THE MICROCIRCULATORY SYSTEM OF PERIOVULATORY OVINE FOLLICLES
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DOI：
10.1095/biolreprod39.4.989
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发表时间：
1988-11-01
影响因子：
3.6
通讯作者：
MURDOCH, WJ
中科院分区：
文献类型：
--
作者：
CAVENDER, JL;MURDOCH, WJ
利用光镜、透射电镜和扫描电镜对母羊排卵期鞘膜血管组织和血细胞进行了研究。促黄体激素在排卵前激增后，血管管腔的横截面积增加，在排卵前下降，随着黄体的形成，血管管腔的横截面积又增加。单位面积鞘组织的血管数量在排卵前下降，在黄体化过程中增加。卵泡柱头在排卵期附近发育，完全没有血管。这些发现与血管舒张、血管通透性增加、组织水肿、充血、缺血、血管损伤和血管生成的证据在时间上是一致的。中性粒细胞和嗜酸性粒细胞在排卵前迁出血管腔室。在排卵期前后，鞘膜组织中有大量外渗血，大量血小板粘附在受损的血管内皮上。排卵时单核/巨噬细胞明显外溢。在排卵期和排卵期后的卵泡中，淋巴细胞常沿内皮边缘排列。在黄体化过程中，嗜碱性细胞的积累与新毛细血管的发育有关。我们的观察结果与排卵泡周围过程包括急性炎症、组织损伤、腺体转化和愈合的概念一致。
Thecal vascular tissue and blood cells were studied by light, transmission, and scanning electron microscopy during ovulation in the ewe. Cross-sectional areas of vascular lumens increased after the preovulatory surge in luteinizing hormone, decreased before ovulation, and then increased again as corpora lutea formed. Numbers of blood vessels per unit area of thecal tissue declined just before ovulation, then increased during luteinization. The follicular stigma that develops near the time of ovulation was completely void of blood vessels. These findings were paralleled chronologically by evidence of vasodilation, increased vascular permeability, tissue edema, congestion, ischemia, vascular injury, and angiogenesis. Neutrophils and eosinophils migrated out of the vascular compartment before ovulation. Around the time of ovulation, there were masses of extravasated blood in thecal tissue, and numerous platelets adhered to damaged vascular endothelium. Extravasated monocytes/macrophages were evident for ovulation. Numbers of extravascular lymphocytes remained relatively constant, but the lymphocytes were often marginated along endothelium in ovulatory and postovulatory follicles. Basophilic cells accumulated in association with the development of new capillaries during luteinization. Our observations are consistent with the concept that periovulatory follicular processes include acute inflammation, tissue damge, glandular transformation, and healing.