Caenorhabditis elegans DLC-1 associates with ribonucleoprotein complexes to promote mRNA regulation.
Caenorhabditis elegans DLC-1 associates with ribonucleoprotein complexes to promote mRNA regulation.
复制标题
秀丽隐杆线虫 DLC-1 与核糖核蛋白复合物结合以促进 mRNA 调节。
DOI：
10.1002/1873-3468.13259
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发表时间：
2018
期刊：
影响因子：
3.5
通讯作者：
Voronina,Ekaterina
中科院分区：
文献类型：
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作者：
Day,NicholasJ;Ellenbecker,Mary;Voronina,Ekaterina
核糖核蛋白复合体包含mRNAs及其调节蛋白，对基因表达进行转录后调控。许多RNA结合蛋白的功能依赖于它们与辅因子的结合。在这里，我们使用基因组方法来鉴定与DLC-1相关的转录本，DLC-1是一种蛋白质，以前被鉴定为两种不相关的RNA结合蛋白的辅助因子，这两种蛋白质在秀丽线虫胚系中发挥作用。在2732个潜在的DLC-1靶点中，大多数是与卵子发生相关的生殖系mRNAs。去除DLC-1会影响其在卵母细胞中表达的靶分子Meg-1和meg-3。我们认为DLC-1是多种核糖核蛋白复合体的辅助因子，包括在卵子发生过程中调节基因表达的那些复合体。
Ribonucleoprotein complexes, which contain mRNAs and their regulator proteins, carry out post‐transcriptional control of gene expression. The function of many RNA‐binding proteins depends on their association with cofactors. Here, we use a genomic approach to identify transcripts associated with DLC‐1, a protein previously identified as a cofactor of two unrelated RNA‐binding proteins that act in theCaenorhabditis elegansgermline. Among the 2732 potential DLC‐1 targets, most are germline mRNAs associated with oogenesis. Removal of DLC‐1 affects expression of its targets expressed in the oocytes,meg‐1andmeg‐3. We propose that DLC‐1 acts as a cofactor for multiple ribonucleoprotein complexes, including the ones regulating gene expression during oogenesis.