Effect of high fluoride concentration on bovine dentin demineralization in narrow grooves in vitro.
Effect of high fluoride concentration on bovine dentin demineralization in narrow grooves in vitro.
复制标题
高氟浓度对体外牛牙本质窄沟脱矿的影响。
DOI：
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发表时间：
1999
影响因子：
1.9
通讯作者：
J. M. T. Cate
中科院分区：
文献类型：
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作者：
E. Zaura;R. Exterkate;J. M. T. Cate
以前，牙本质沟槽模型被开发用于研究牙菌斑保留部位的龋齿，如边缘间隙、凹坑和裂缝。本研究的目的是研究氟化物对牙本质沟槽深度的体外脱矿影响，并与新制备的树脂改性玻璃离子水泥（RMGIC）进行比较，RMGIC是高氟释放源。在20只牛冠状牙本质盘的唇表面切割3个平行凹槽（280微米宽x 500微米深）。在10个椎间盘中，第一个凹槽和椎间盘边缘之间的表面积（约6 mm2）被RMGIC覆盖。圆盘暴露在酸化甲基纤维素凝胶系统中1或2周。通过横向显微放射分析获得矿物含量的变化。结果显示，RMGIC组的综合矿物质损失（IML）减少了60%，尽管RMGIC对损伤深度没有显著影响。各组间的脱矿模式不同：RMGIC组的表面下病变在沟底的IML没有与对照组相同程度的减少。结果表明，高剂量氟化物可抑制矿物质流失，但不影响病变深度。RMGIC组中病变随深度的均匀性可能是由于氟化物引起的凹槽入口处脱矿的抑制。
Previously, a dentin groove model was developed to investigate caries at plaque retention sites, such as marginal gaps, and pits and fissures. The aim of the present study was to investigate the in vitro effect of fluoride on the demineralization of dentin in the depth of the grooves at various distances from a freshly prepared resin-modified glass ionomer cement (RMGIC), which was used as the source of high fluoride release. Three parallel grooves (280 microm wide x 500 microm deep) were cut in the labial surface of 20 bovine coronal dentin discs. In 10 discs, the surface area (approximately 6 mm2) between the first groove and the edge of the disc was covered with RMGIC. The discs were exposed to an acidified methylcellulose gel system for 1 or 2 wk. Changes in mineral content were obtained by transverse microradiographic analysis. The results showed that integrated mineral loss (IML) was reduced up to 60% in the RMGIC groups, although the lesion depth was not significantly influenced by RMGIC. The pattern of demineralization differed between groups: the subsurface lesions in RMGIC groups did not decrease in IML towards the base of the groove to the same degree as in the control groups. It is concluded that high amount of fluoride inhibits loss of mineral, while not affecting the lesion depth. The homogeneity of the lesions with depth in the RMGIC group may be explained by a fluoride-induced inhibition of demineralization at the entrance of the grooves.