Transport of 14C-lableled indoleacetic acid in Vicia root segments
Transport of 14C-lableled indoleacetic acid in Vicia root segments
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14C 标记的吲哚乙酸在蚕豆根段中的转运
DOI：
10.1093/oxfordjournals.pcp.a075700
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发表时间：
1978
影响因子：
4.9
通讯作者：
Y. Ohwaki
中科院分区：
文献类型：
--
作者：
S. Tsurumi;Y. Ohwaki
研究了IAA在根段中的转运情况，并与完整根中的转运情况进行了比较。将含有IAA-2-14C的羊毛脂膏（1mm宽的环）或琼脂块（3×3×1.5mm）分别涂于根段的表面或切割端；将transported14C收集到放置在片段切割末端的受体琼脂块中。当羊毛脂膏作用于5-mm节段时，IAA的基端转运比端端转运占优势。当琼脂块应用于1-和2-mm片段时，也是如此；然而，在较长的节段（3和5毫米长）中，最初主要发生基顶运动，但在2-3小时后被顶尖运动所超越。在离茎尖2 ~ 4、4 ~ 6和8 ~ 10 mm的区域，最顶端的部分表现出明显的基部运动优势。14c的顶端和基端运动速度分别为3 ~ 3.8 mm/hr和8 ~ 12 mm/hr。放射自显像研究和通过节段中心纵向插入金属丝的实验表明，基端运动主要通过根的外侧发生，而尖端运动主要通过中心柱发生。本研究结果与先前完整根的结果一致。描述了极性运动的一些特性，如其特异性、TIBA的抑制作用和对条件温度的依赖性。
IAA transport inViciaroot segments was investigated for comparison with that in intact roots. Lanolin paste (1-mm-wide ring) or agar blocks (3×3×1.5mm), both containing IAA-2-14C were applied to the surface or a cut end of the root segments, respectively; transported14C was collected in receiver agar blocks placed on the cut end of the segments. When lanolin paste was applied to 5-mm segments, basipetal transport of IAA predominated over acropetal transport. When agar blocks were applied to 1- and 2-mm segments, the same was true; in longer segments (3 and 5 mm long), however, basipetal movement occurred predominantly at first but was surpassed by acropetal movement after 2–3 hr. Among the segments tested (regions 2–4, 4–6 and 8–10 mm from the tip), the most apical one showed the distinctest predominancy of basipetal movement. The velocities of the acropetal and basipetal movement of the14C were estimated at 3–3.8 and 8–12 mm/hr, respectively. Autoradiographic study and the experiment in which wire was inserted longitudinally through the central part of the segments showed that basipetal movement occurred mainly through the outer part of the roots and acropetal movement mainly through the central cylinder. The present results were compatible with those obtained previously with intact roots. Some properties of polar movement, such as its specificity, inhibition by TIBA, and dependency on tern eprature are described.