Supragingival Plaque Microbiome Ecology and Functional Potential in the Context of Health and Disease.
Supragingival Plaque Microbiome Ecology and Functional Potential in the Context of Health and Disease.
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DOI：
10.1128/mbio.01631-18
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发表时间：
2018-11-27
期刊：
影响因子：
6.4
通讯作者：
Dupont CL
中科院分区：
文献类型：
--
作者：
Espinoza JL;Harkins DM;Torralba M;Gomez A;Highlander SK;Jones MB;Leong P;Saffery R;Bockmann M;Kuelbs C;Inman JM;Hughes T;Craig JM;Nelson KE;Dupont CL
口腔健康具有重要的经济意义，在美国每年花费超过1000亿美元用于牙齿护理。微生物组在口腔健康中起着至关重要的作用，但仍然分类不清。为了解决儿童口腔中微生物多样性和功能如何与龋齿诊断相关的问题，我们调查了44对青少年双胞胎的龈上菌斑生物膜微生物组。使用鸟枪测序，我们构建了一个描述核心龈上菌斑微生物组的基因组百科全书。这揭示了口腔微生物组中几种新的先前未表征但普遍存在的微生物谱系。龋齿是一种微生物群落代谢紊乱，不能用单一病因学来描述，我们的研究结果为下一代龋齿诊断工具和治疗方法提供了所需的信息。为了解决儿童口腔中微生物多样性和功能如何与龋齿诊断相关的问题，我们调查了44对青少年双胞胎的龈上菌斑生物膜微生物组。使用鸟枪测序，我们构建了一个描述核心龈上菌斑微生物组的基因组百科全书。龋齿表型在特定基因组丰度和不同的社区组成概况，包括应变水平的变化，统计学上显着富集。与龋齿统计学相关的代谢途径包括几种糖相关磷酸转移酶系统、抗菌素耐药性和金属转运。许多密切相关的先前未表征的微生物在中心代谢中有很大的变化，包括导致营养缺陷的生物合成途径的丧失，改变了生态作用。我们还描述了来自人类微生物组的第一个完整的Gracilibacteria基因组。龋齿是一种微生物群落代谢紊乱，不能用单一病因学来描述，我们的研究结果为下一代龋齿诊断工具和治疗方法提供了所需的信息。
Oral health has substantial economic importance, with over $100 billion spent on dental care in the United States annually. The microbiome plays a critical role in oral health, yet remains poorly classified. To address the question of how microbial diversity and function in the oral cavities of children relate to caries diagnosis, we surveyed the supragingival plaque biofilm microbiome in 44 juvenile twin pairs. Using shotgun sequencing, we constructed a genome encyclopedia describing the core supragingival plaque microbiome. This unveiled several new previously uncharacterized but ubiquitous microbial lineages in the oral microbiome. Caries is a microbial community metabolic disorder that cannot be described by a single etiology, and our results provide the information needed for next-generation diagnostic tools and therapeutics for caries. To address the question of how microbial diversity and function in the oral cavities of children relates to caries diagnosis, we surveyed the supragingival plaque biofilm microbiome in 44 juvenile twin pairs. Using shotgun sequencing, we constructed a genome encyclopedia describing the core supragingival plaque microbiome. Caries phenotypes contained statistically significant enrichments in specific genome abundances and distinct community composition profiles, including strain-level changes. Metabolic pathways that are statistically associated with caries include several sugar-associated phosphotransferase systems, antimicrobial resistance, and metal transport. Numerous closely related previously uncharacterized microbes had substantial variation in central metabolism, including the loss of biosynthetic pathways resulting in auxotrophy, changing the ecological role. We also describe the first complete Gracilibacteria genomes from the human microbiome. Caries is a microbial community metabolic disorder that cannot be described by a single etiology, and our results provide the information needed for next-generation diagnostic tools and therapeutics for caries.