The Oral Microbiome and Lung Cancer Risk: An Analysis of 3 Prospective Cohort Studies.
The Oral Microbiome and Lung Cancer Risk: An Analysis of 3 Prospective Cohort Studies.
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口腔微生物组和肺癌风险：3 项前瞻性队列研究的分析。
DOI：
10.1093/jnci/djac149
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
BeaneFr
中科院分区：
文献类型：
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作者：
Vogtmann,Emily;Hua,Xing;Yu,Guoqin;Purandare,Vaishnavi;Hullings,AutumnG;Shao,Dantong;Wan,Yunhu;Li,Shilan;Dagnall,CaseyL;Jones,Kristine;Hicks,BelyndaD;Hutchinson,Amy;Caporaso,JGregory;Wheeler,William;Sandler,DaleP;BeaneFr
BackgroundPrevious studies suggested associations between the oral microbiome and lung cancer.MethodsUsing a case-cohort design，1306 incident lung cancer cases were identified in the Agricultural Health Study; National Institutes of Health-AARP Diet and Health Study; and Prostate，Lung，Colorectal，and Ovarian Cancer Screening Trial.按年龄、性别和吸烟史分层随机选择参考亚组。采用DSP DNA病毒病原体试剂盒从口腔洗液标本中提取DNA，扩增16 S rRNA基因V4区并测序，利用QIIME 2进行生物信息学分析。使用加权考克斯比例风险models.ResultsHigher α多样性与较低的肺癌风险（香农指数风险比= 0.90，95%置信区间= 0.84至0.96）的风险比和95%置信区间进行了计算。微生物群落的特定主成分向量也与肺癌风险统计学显著相关。经多重检验调整后，3个属的相对丰度越大，1个属的存在与肺癌风险越高相关，而3个属的存在与肺癌风险较低相关。例如，链球菌丰度每增加一个SD，患肺癌的风险就增加1.14倍（95%可信区间= 1.06 - 1.22）。鳞状细胞癌病例和前smokers.ConclusionsMultiple口腔微生物措施之间的关联是最强的前瞻性与肺癌的风险在3个美国队列研究，吸烟史和组织学亚型的协会不同。口腔微生物组可能为肺癌预防提供新的机会。
BackgroundPrevious studies suggested associations between the oral microbiome and lung cancer, but studies were predominantly cross-sectional and underpowered.MethodsUsing a case-cohort design, 1306 incident lung cancer cases were identified in the Agricultural Health Study; National Institutes of Health-AARP Diet and Health Study; and Prostate, Lung, Colorectal, and Ovarian Cancer Screening Trial. Referent subcohorts were randomly selected by strata of age, sex, and smoking history. DNA was extracted from oral wash specimens using the DSP DNA Virus Pathogen kit, the 16S rRNA gene V4 region was amplified and sequenced, and bioinformatics were conducted using QIIME 2. Hazard ratios and 95% confidence intervals were calculated using weighted Cox proportional hazards models.ResultsHigher alpha diversity was associated with lower lung cancer risk (Shannon index hazard ratio = 0.90, 95% confidence interval = 0.84 to 0.96). Specific principal component vectors of the microbial communities were also statistically significantly associated with lung cancer risk. After multiple testing adjustment, greater relative abundance of 3 genera and presence of 1 genus were associated with greater lung cancer risk, whereas presence of 3 genera were associated with lower risk. For example, every SD increase inStreptococcusabundance was associated with 1.14 times the risk of lung cancer (95% confidence interval = 1.06 to 1.22). Associations were strongest among squamous cell carcinoma cases and former smokers.ConclusionsMultiple oral microbial measures were prospectively associated with lung cancer risk in 3 US cohort studies, with associations varying by smoking history and histologic subtype. The oral microbiome may offer new opportunities for lung cancer prevention.