Mechanically Tailored Agarose Hydrogels through Molecular Alloying with β-Sheet Polysaccharides
Mechanically Tailored Agarose Hydrogels through Molecular Alloying with β-Sheet Polysaccharides
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DOI：
10.1002/marc.201400353
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发表时间：
2015-01-01
影响因子：
4.6
通讯作者：
Shastri, V. Prasad
中科院分区：
文献类型：
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作者：
Forget, Aurelien;Pique, Raphaelle-Anne;Shastri, V. Prasad
越来越多的证据表明，组织的机械特性提供了控制细胞命运的重要线索。然而，由于与交联化学相关的挑战，具有可调物理化学性质的水凝胶的实现受到限制。最近的研究表明，机械上定义明确的可注射多糖水凝胶可以通过将其二级结构从α -螺旋转换为β -片来设计。在此基础上，提出了一种通过与富含-薄片的羧基衍生物共混来调整琼脂糖水凝胶力学性能的新概念。使用这种简单的策略，可以制定具有可预测的粗糙度，纤维组织和剪切模量范围为0.1至100kpa的凝胶。水凝胶的力学性能可以在体内精确地定制，而无需依赖化学反应，有望在从头组织工程中实现力学生物学范式方面发挥重要作用。
There is mounting evidence that the mechanical property of tissues provides important cues that control cell fate. However, implementation of hydrogels with tunable physicochemical properties is limited due to the challenges associated with crosslinking chemistries. It has been recently shown that mechanically well-defined injectable polysaccharide hydrogels can be engineered by switching their secondary structure from an alpha-helix to a beta-sheet. Based on these findings, a new concept is presented to tailor the mechanical properties of agarose hydrogels via the blending with the beta-sheet-rich carboxylated derivative. Using this simple strategy, gels with predictable roughness, fiber organization, and shear modulus ranging from 0.1 to 100 kPa can be formulated. Hydrogels whose mechanical properties can be precisely tailored in vivo without the recourse for chemical reactions are expected to play an important role in implementing mechanobiology paradigms in de novo tissue engineering.