Biliary proteins and their redox status changes in gallstone patients.
Biliary proteins and their redox status changes in gallstone patients.
复制标题
胆结石患者的胆汁蛋白及其氧化还原状态的变化。
DOI：
10.1111/j.1365-2362.2009.02187.x
复制
发表时间：
2009
影响因子：
5.5
通讯作者：
Portincasa,P
中科院分区：
文献类型：
--
作者：
Grattagliano,I;Wang,DQ-H;DiCiaula,A;Diogo,CV;Palasciano,G;Portincasa,P
背景蛋白质可能是胆汁中胆固醇结晶的原核剂。然而，很少有人知道的氧化还原状态的胆汁蛋白质在人类和他们的相互作用与结晶的胆汁cholesterol.Materials和methods胆囊胆汁获得胆囊切除术从86症状患者胆固醇结石（32多发性和32孤立性结石）或色素结石（n= 22），并研究蛋白质氧化还原状态[羰基和巯基（PSH）浓度]，总脂质和蛋白质水平以及胆固醇饱和指数（CSI）。结果胆固醇结石患者的胆固醇晶体首次出现时间（COT）明显缩短（3d vs.>21 d，P<0·05），胆固醇结石患者的胆固醇晶体首次出现时间（149 ± 10% vs. 97 ± 7%，P <0·05）和总胆汁蛋白（1·96 ± 0·1 mg mL− 1 vs. 0.55 ± 0.1 mg mL−1，P<0.05）。 胆固醇结石患者的蛋白质巯基浓度显著较低（P<0·05）（18 ± 4 nmol mg− 1蛋白质vs. 49 ± 16 nmol mg− 1蛋白质），而胆固醇和色素结石患者的总脂质和羰基蛋白浓度相似。 结石的数量/类型（多发性>孤立性>色素性结石，P= 0.009）和总蛋白浓度（高水平>低水平，P= 0.004）影响结石的结晶概率。 COT与总蛋白含量呈负相关（r=-0.45，P = 0.03.ConclusionsBiles with cholesterol stones showed high CSI and total protein concentration，and rapid COT，which is even faster in patients with multiple stones and high protein concentration. 胆固醇结石患者的低PSH水平表明生化转变，可能会影响胆固醇结晶。
AbstractBackgroundProteins might act as pronucleating agents of cholesterol crystallization in bile. However, little is known about the redox status of biliary proteins in humans and their interaction with crystallization of biliary cholesterol.Materials and methodsGallbladder biles were obtained at cholecystectomy from 86 symptomatic patients with either cholesterol gallstones (32 multiple and 32 solitary stones) or pigment stones (n= 22), and studied for protein redox status [carbonyl and sulfhydryl (PSH) concentrations], total lipid and protein levels and cholesterol saturation index (CSI). First appearance of cholesterol crystals in ultrafiltered bile (crystal observation time, COT) was studied with polarizing light microscopy during 21 days.ResultsPatients with cholesterol stones had significantly shorter COT (3 days vs. >21 days,P< 0·05), higher CSI (149 ± 10% vs. 97 ± 7%,P< 0·05) and higher total biliary proteins (1·96 ± 0·1 mg mL−1vs. 0·55 ± 0·1 mg mL−1,P< 0·05) than patients with pigment stones. Patients with cholesterol stones had significantly lower (P< 0·05) level of protein sulfhydryl concentrations (18 ± 4 nmol mg−1protein vs. 49 ± 16 nmol mg−1protein), while total lipid and carbonyl proteins concentrations were similar between cholesterol and pigment stone patients. Crystallization probability was influenced by the number/type of gallstones (multiple > solitary > pigment stones,P= 0·009) and total protein concentration (high > low levels,P= 0·004). COT was negatively correlated with total protein content (r= −0·45,P= 0·03).ConclusionsBiles with cholesterol stones show high CSI and total protein concentration, and rapid COT, which is even faster in patients with multiple stones and high protein concentration. Low PSH levels in cholesterol stone patients point to a biochemical shift, potentially able to affect cholesterol crystallization.