MOUSE STRAIN DIFFERENCES IN SUSCEPTIBILITY TO CEREBRAL-ISCHEMIA ARE RELATED TO CEREBRAL VASCULAR ANATOMY
MOUSE STRAIN DIFFERENCES IN SUSCEPTIBILITY TO CEREBRAL-ISCHEMIA ARE RELATED TO CEREBRAL VASCULAR ANATOMY
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DOI：
10.1038/jcbfm.1993.87
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发表时间：
1993-07-01
影响因子：
6.3
通讯作者：
WILLETTE, RN
中科院分区：
文献类型：
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作者：
BARONE, FC;KNUDSEN, DJ;WILLETTE, RN
在三种不同品系的小鼠（BDF、CFW 和 BALB/C）中研究了脑缺血的后果。不同菌株对24小时局灶性缺血的易感性存在显着差异。大脑中动脉闭塞 (MCAO) 后，BDF 小鼠的梗塞体积 (mm3) 为 5 +/- 3，CFW 小鼠为 15 +/- 5，BALB/C 小鼠为 23 +/- 3 (p < 0.05)。 MCAO 加上同侧颈总动脉闭塞 (CCAO) 导致 BDF 小鼠的梗死体积为 15 +/- 9，CFW 小鼠为 38 +/- 10，BALB/C 小鼠为 72 +/- 12（p < 0.05）。此外，MCAO 加 CCAO 导致 42% 的 CFW 小鼠和 67% 的 BALB/C 小鼠在 24 小时内死亡，但没有任何 BDF 小鼠死亡（p < 0.05）。单独使用 CCAO 可使 36% 的 BALB/C 小鼠产生多灶性半球梗塞，但其他两种品系小鼠则不会。所有接受假手术的小鼠品系的大脑均未出现任何缺血性损伤。三种小鼠品系的动脉血压、血气和血细胞特征相对相似。然而，脑血管解剖学的炭黑研究显示，与 BDF 小鼠相比，BALB/C 的 Willis 环不完整（即后交通动脉开放的频率显着降低）（p < 0.05），而 CFW 小鼠处于中间状态。基于这些解剖数据，还对 BALB/C 小鼠在双侧 CCAO 产生的短暂性全脑缺血后进行了评估。 BALB/C 小鼠在双侧 CCAO 1 分钟内皮质微血管灌注和 EEG 功率降低 >85%。此外，7 天内海马神经元 CA1 损伤和死亡率与全脑缺血的持续时间相关（p < 0.05）。这些数据表明，小鼠品系之间对脑缺血的敏感性存在显着差异，这似乎至少部分与后交通动脉水平的功能性血管解剖结构有关。我们特别指出 BALB/C 小鼠作为局部和整体缺血的敏感且可重复模型的潜在用途。
The consequences of cerebral ischemia were studied in three different strains (BDF, CFW, and BALB/C) of mice. The different strains exhibited significant differences in susceptibility to 24-h focal ischemia. Following middle cerebral artery occlusion (MCAO), infarct volumes (mm3) were 5 +/- 3 in BDF, 15 +/- 5 in CFW, and 23 +/- 3 in BALB/C mice (p < 0.05). MCAO plus ipsilateral common carotid artery occlusion (CCAO) resulted in infarct volumes of 15 +/- 9 in BDF, 38 +/- 10 in CFW, and 72 +/- 12 in BALB/C mice (p < 0.05). In addition, MCAO plus CCAO produced death by 24 h in 42% of CFW and 67% of BALB/C mice, but not in any BDF mice (p < 0.05). CCAO alone produced multifocal hemispheric infarctions in 36% of BALB/C mice but not in the other two strains. Brains of all mouse strains subjected to sham surgery were free of any ischemic injury. Arterial blood pressures, blood gases, and blood cell profiles were relatively similar for the three mouse strains. However, carbon black studies of the cerebrovascular anatomy revealed an incomplete circle of Willis (i.e., a significant decrease in the frequency of patent posterior communicating arteries) for BALB/C compared with BDF mice (p < 0.05), with CFW mice being intermediary. Based on these anatomical data, BALB/C mice also were evaluated following transient global brain ischemia produced by bilateral CCAO. BALB/C mice exhibited a >85% reduction in cortical microvascular perfusion and EEG power within 1 min of bilateral CCAO. Also, hippocampal neuronal CA1 damage and mortality over 7 days were related to the duration of global brain ischemia (p < 0.05). These data demonstrate a significant difference between mouse strains in their sensitivity to cerebral ischemia that appears to be related, at least in part, to the functional vascular anatomy at the level of the posterior communicating arteries. In particular, we point out the potential usefulness of BALB/C mice as a sensitive and reproducible model of focal and global ischemia.