Evidence for the role of oxidative stress in the acetylation of histone H3 by ethanol in rat hepatocytes.
Evidence for the role of oxidative stress in the acetylation of histone H3 by ethanol in rat hepatocytes.
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DOI：
10.1016/j.alcohol.2010.06.003
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发表时间：
2010-09
期刊：
影响因子：
--
通讯作者：
Shukla SD
中科院分区：
文献类型：
--
作者：
Choudhury M;Park PH;Jackson D;Shukla SD
乙醇诱导的氧化应激与组蛋白 H3 赖氨酸 9 (H3AcK9) 乙酰化之间的关系仍不清楚，因此在大鼠肝细胞的原代培养物中进行了研究。用乙醇和一组选定的药物处理细胞，并监测 H3AcK9 和活性氧 (ROS) 的状态。当肝细胞在 N-乙酰半胱氨酸（ROS 还原剂）或膳食抗氧化剂（槲皮素、白藜芦醇）或 NADPH 氧化酶抑制剂夹竹桃麻素存在下暴露于乙醇（50 mM，24 小时）时，乙醇诱导的 ROS 和 H3AcK9 增加，两者均显着减少。另一方面，L-丁硫氨酸-亚砜亚胺（ROS 诱导剂）和线粒体复合物 I（鱼藤酮）和 III（抗霉素）抑制剂可增加乙醇诱导的 H3AcK9（p<0.01）。氧化应激还影响乙醇诱导的乙醇脱氢酶 1 (ADH1) mRNA 表达。这些结果首次证明氧化应激参与乙醇诱导的肝细胞中组蛋白 H3 乙酰化。
The relationship between ethanol induced oxidative stress and acetylation of histone H3 at lysine 9 (H3AcK9) remains unknown and was therefore investigated in primary cultures of rat hepatocytes. Cells were treated with ethanol and a select group of pharmacological agents and the status of H3AcK9 and reactive oxygen species (ROS) were monitored. When hepatocytes were exposed to ethanol (50 mM, 24 hr) in the presence of N-acetyl cystein (ROS reducer) or dietary antioxidants (quercetin, resveratrol), or NADPH oxidase inhibitor apocynin, ethanol induced increases in ROS and H3AcK9, both were significantly reduced. On the other hand, l-buthionine-sulfoximine (ROS inducer) and inhibitor of mitochondrial complex I (rotenone) and III (antimycin) increased ethanol induced H3AcK9 (p<0.01). Oxidative stress also affected ethanol induced alcohol dehydrogenase 1 (ADH1) mRNA expression. These results demonstrate for the first time that oxidative stress is involved in the ethanol induced histone H3 acetylation in hepatocytes.
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DOI：
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发表时间：
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影响因子：
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作者：
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