A simplified procedure for synthesizing large quantities of highly purified uridine [beta-32P]diphospho-N-acetylglucosamine.
A simplified procedure for synthesizing large quantities of highly purified uridine [beta-32P]diphospho-N-acetylglucosamine.
复制标题
用于合成大量高纯度尿苷 [β-32P]二磷酸-N-乙酰氨基葡萄糖的简化程序。
DOI：
10.1016/0003-2697(84)90163-5
复制
发表时间：
1984
影响因子：
2.9
通讯作者：
Kornfeld,S
中科院分区：
文献类型：
--
作者：
Lang,L;Kornfeld,S
介绍了一种合成[β-32P] udp - n -乙酰氨基葡萄糖的简化方法。这种新方法利用商业上可用的酶、化学乙酰化和制备薄层色谱，可用于以极高的比活性和纯度合成毫比里量的糖核苷酸。
A simplified procedure for the synthesis of [β-32P]UDP-N-acetylglucosamine is described. This novel method utilizes commericially available enzymes, chemical acetylation, and preparative thin-layer chromatography and can be used to synthesize millicurie quantities of the sugar nucleotide at an extremely high specific activity and purity.