Genetic diversity of vaginal lactobacilli from women in different countries based on 16S rRNA gene sequences
Genetic diversity of vaginal lactobacilli from women in different countries based on 16S rRNA gene sequences
复制标题
DOI：
10.1046/j.1365-2672.2002.01547.x
复制
发表时间：
2002-01-01
影响因子：
4
通讯作者：
Tao, L
中科院分区：
文献类型：
--
作者：
Pavlova, SI;Kilic, AO;Tao, L
目的：乳酸杆菌广泛分布于食品和环境中，有的以肠道细菌的形式在人体内定植。本研究的目的是确定种乳酸杆菌定植阴道，并与那些在食品和environment.Methods和结果：35乳杆菌菌株从7个国家的妇女进行了分离，并从16S rRNA基因序列进行了测定，并与现有的数据在GenBank中进行比较。基于1465个核苷酸的分析，使用邻接法构建了系统发育树。结果表明，阴道分离株以L. n.皱叶藓jensenii和L.加塞里有些是L。瓦格利湖发酵L. mucosae，L. paracasei和L.鼠李糖两个分离物从一个美洲土著妇女显示不同的分支，表明新的peptides类型。很少有阴道分离物与食物或环境中的乳杆菌属菌种相匹配。n.皱叶藓jensenii和L.研究的意义和影响：了解阴道乳杆菌物种丰富度和在全世界妇女中的分布可能导致设计更好的益生菌产品作为细菌替代疗法。
Aims: Lactobacilli are widely distributed in food and the environment, and some colonize the human body as commensal bacteria. The aim of this study was to determine the species of lactobacilli that colonize the vagina and compare them with those found in food and the environment.Methods and Results: Thirty-five Lactobacillus strains from women from seven countries were isolated, and sequences from 16S rRNA genes were determined and compared with existing data in GenBank. A phylogenetic tree was achieved using the Neighbour-joining method based on the analysis of 1465 nucleotides. The results showed that most vaginal isolates were L. crispatus, L. jensenii and L. gasseri. Some were L. vaginalis, L. fermentum, L. mucosae, L. paracasei and L. rhamnosus. Two isolates from a native American woman displayed distinct branches, indicating novel phylotypes. Few vaginal isolates matched food or environmental Lactobacillus species.Conclusions: Most women worldwide were colonized by three common Lactobacillus species: L. crispatus, L. jensenii and L. gasseri.Significance and Impact of Study: Knowledge of vaginal Lactobacillus species richness and distribution in women worldwide may lead to the design of better probiotic products as bacterial replacement therapy.