Self‐Nonself Recognition Activity Extracted from Self‐Sterile Eggs of the Ascidian, Ciona intestinalis
Self‐Nonself Recognition Activity Extracted from Self‐Sterile Eggs of the Ascidian, Ciona intestinalis
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海鞘自育卵提取的自我-非自我识别活性
DOI：
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发表时间：
1991
期刊：
影响因子：
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通讯作者：
M. Nakauchi
中科院分区：
文献类型：
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作者：
K. Kawamura;Maki Nomura;Tomoko Kameda;Hiroko Shimamoto;M. Nakauchi
用酸性海水(pH 3.2)洗涤两性自生无菌海鞘的卵，然后将洗涤液调节至pH 8.2。该溶液仅能抑制非自体精子与卵子卵黄膜(Vc)的结合，表明其具有自我非自我识别活性。该酶具有热稳定性，对胰酶不敏感，但可被V-8蛋白酶和α-葡萄糖苷酶破坏。提取物的冻干粉的疏水和亲水成分都显示出异体识别活性。在薄层层析上，疏水组分给出了主要的葡萄糖斑点(GLC)和主要含有谷氨酸和/或谷氨酰胺的多肽斑点(S)。用高效液相色谱法对葡糖结合物进行了纯化，结果表明它能不同程度地阻断精子与卵子的结合。单独的多肽亚组分没有抑制活性，但它们组合在一起显示出抑制活性。这些发现表明，乔纳卵的酸提取物含有富含GLC的精卵结合的非特异性抑制物，这可能是自交不亲和的主要影响因素，以及富含GLX的调节剂，它们是同种异体精子的受体。这些组分的协同作用可能是导致乔纳配子异源识别多样性的原因。
Eggs of the hermaphrodite, self‐sterile ascidian, Ciona intestinalis, were washed with acid seawater (pH 3.2), and the washing solution was then adjusted to pH 8.2. This solution was found to inhibit only the binding of non‐autologous sperm to the vitelline coat (VC) of eggs, indicating that it contained self‐nonself recognition activity. This activity was heat‐stable and insensitive to trypsin, but was destroyed by V‐8 protease and α‐glucosidase. Both the hydrophobic and hydrophilic components of a lyophilized powder of the extract showed allo‐recognizing activity. On TLC, the hydrophobic components gave a major spot of glucose (Glc) and a peptide spot(s) containing mainly glutamic acid and/or glutamine (Glx). The glucosyl conjugate was purified by HPLC and shown to block sperm‐egg binding to various extents. Individual peptide subfractions had no inhibitory activity, but in combination they showed inhibitory activity. These findings suggest that the acid extract of Ciona eggs contains a Glc‐enriched nonspecific inhibitor of sperm‐egg binding, which could be the primary effector of self‐incompatibility, and Glx‐enriched modulators, which serve as acceptors of allo‐sperm. The cooperative interactions of these components may be responsible for the diversity of allo‐recognition in Ciona gametes.
CD8 分子与细胞毒性 T 细胞上的 T 细胞受体复合物之间存在关联的证据。
DOI：
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发表时间：
1987
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
Takada,S;Engleman,EG
通讯作者：
Engleman,EG