The fgr gene responsible for rice fragrance was restricted within 69 kb
The fgr gene responsible for rice fragrance was restricted within 69 kb
复制标题
DOI：
10.1016/j.plantsci.2006.05.013
复制
发表时间：
2006-10-01
期刊：
影响因子：
5.2
通讯作者：
Xu, Mingliang
中科院分区：
文献类型：
--
作者：
Chen, Saihua;Wu, Juan;Xu, Mingliang
在100多种挥发性风味成分中，2-乙酰-1-吡咯烷(2AP)是巴西香米和茉莉花香米的关键香气成分。已有报道8号染色体上的一个单隐性基因(FGR)负责2AP的产生。我们利用SSR标记进行的初步定位工作证实了先前的定位结果，并将FGR基因座定位在RM8264和RM3459之间，物理距离接近800kb。然后，FGR区域被高密度标记饱和，这些标记是通过利用水稻亚种间的序列差异而开发的。与2891个个体组成的分离群体作图后，FGR基因座被限制在69kb的区间内，左侧标记L02和右侧标记L06。此外，通过对关键重组子及其后代的两种基因型和2AP水平的同步调查，确定了FGR基因座。FGR区序列分析显示，3个候选基因分别编码真核型碳酸氢酶、3-甲基巴豆酸辅酶A羧基酶β链和甜菜碱醛脱氢酶。本研究结果为FGR基因的克隆和分子标记辅助选择奠定了基础。(C)2006爱思唯尔爱尔兰有限公司。保留所有权利。
From more than 100 volatile flavor components, 2-acetyl-1-pyrroline (2AP) stands out as the key component of aroma in both basmati- and jasmine-style fragrant rice. It has been reported that a single recessive gene (fgr) on chromosome 8 is responsible for the production of 2AP. Our initial mapping efforts with SSR markers confirmed the previous mapping result, and placed the fgr locus between RM8264 and RM3459 with the physical distance of similar to 800 kb. The fgr region was then saturated with high-density markers developed by exploiting sequence diversities between indica and japonica rice subspecies. After mapping with segregant populations consisting of totally 2891 individuals, the fgr locus was restricted to an interval of 69 kb flanked by the left marker L02 and the right marker L06. Furthermore, the fgr locus was confirmed by simultaneous investigation of both genotypes and 2AP levels for the key recombinants and their offspring. Sequence analysis of the fgr region revealed three candidate genes encoding respective eukaryotic-type carbonic anhydrase, 3-methylcrotonyt-CoA carboxylase beta chain, and betaine aldehyde dehydrogenase. The result presented here would pave the way for both cloning and marker-assisted selection of the fgr gene. (c) 2006 Elsevier Ireland Ltd. All rights reserved.