IDENTIFICATION OF NEW SCHISTOSOMA-MANSONI GENES BY THE EST STRATEGY USING A DIRECTIONAL CDNA LIBRARY
IDENTIFICATION OF NEW SCHISTOSOMA-MANSONI GENES BY THE EST STRATEGY USING A DIRECTIONAL CDNA LIBRARY
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DOI：
10.1016/0378-1119(94)00747-g
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发表时间：
1995-01-23
期刊：
影响因子：
3.5
通讯作者：
PENA, SDJ
中科院分区：
文献类型：
--
作者：
FRANCO, GR;ADAMS, MD;PENA, SDJ
以曼氏血吸虫成虫RNA为模板构建定向筛选cDNA文库，用于表达序列标签（expressedsequencetags，EST）的产生。从429个不同的cDNA克隆的一端或两端获得607个EST。其中，只有16%是以前已知的Sm基因。超过22%的克隆与数据库中其他生物的条目相匹配。这些新的Sm基因构成了广泛的转录本，分布在细胞质结构和调节蛋白，酶，膜，核和分泌蛋白，以及具有其他功能的蛋白质。几乎33%的克隆没有显著的数据库匹配，因此可能代表Sm特异性基因。在后者中，几个克隆，判断其在图书馆的冗余，似乎代表丰富的成绩单。这些数据，作为一个整体，超过一倍的Sm基因的核苷酸测序确定的数量，并表明通过基因组测序策略的潜在价值的快速增长的知识复杂的致病生物体。
A directional size-selected cDNA library constructed from Schistosoma mansoni (Sm) adult worm RNA was used for the generation of expressed sequence tags (EST). From one or both ends of 429 distinct cDNA clones 607 EST were obtained. Of these, only 16% were previously known Sm genes. More than 22% of the clones had matches with entries for other organisms in the databases. These new Sm genes constituted a broad range of transcripts distributed among cytoplasmic structural and regulatory proteins, enzymes, membrane, nuclear and secretory proteins, and proteins with other functions. Almost 33% of the clones had no significant database matches and thus potentially represent Sm-specific genes. Among the latter, several clones, as judged by their redundancy in the library, appear to represent abundant transcripts. The data, taken as a whole, more than double the number of Sm genes identified by nucleotide sequencing and indicate the potential value of the adoption of genome sequencing strategies for the rapid increase in knowledge of complex disease-causing organisms.