Influence of the Adjuvants and Genetic Background on the Asthma Model Using Recombinant Der f 2 in Mice.
Influence of the Adjuvants and Genetic Background on the Asthma Model Using Recombinant Der f 2 in Mice.
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DOI：
10.4110/in.2013.13.6.295
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发表时间：
2013-12
期刊：
影响因子：
6
通讯作者：
Cho SH
中科院分区：
文献类型：
--
作者：
Chang YS;Kim YK;Jeon SG;Kim SH;Kim SS;Park HW;Min KU;Kim YY;Cho SH
Der f 2是屋尘螨（Dermatophagoides farinae）的第2组主要过敏原，其功能最近被提出。为了确定重组Der f 2（rDer f 2）在小鼠哮喘模型中的最佳致敏条件，我们在BALB/c和C57 BL/6小鼠中比较了不同佐剂的有效性。在第1天和第14天通过腹腔注射或皮下注射rDer f 2致敏来自两个品系的小鼠。剂量为20 µg。使用具有百日咳毒素（FP）的弗氏佐剂或单独的明矾作为佐剂。在第28、29和30天，用0.1% rDer f 2鼻内攻击小鼠。我们评估了气道高反应性、肺灌洗液中嗜酸性粒细胞比例、气道炎症和血清过敏原特异性抗体反应。未处理的小鼠用作对照。在使用FP的C57 BL/6和使用明矾的BALB/c中，气道高反应性增加（PC 200：13.5±6.3，13.2±6.7对>50 mg/ml，p<0.05）。所有组中嗜酸性粒细胞比例均增加; FP组C57 BL/6、FP组BALB/c、明矾组C57 BL/6、明矾组BALB/c（24.8±3.6、20.3±10.3、11.0±6.9、5.7±2.8，对比0.0± 0.0%，p<0.05）。使用FP或明矾的C57 BL/6血清过敏原特异性IgE水平升高（OD：0.8±1.4，1.1±0.8，vs. 0.0±0.0）。C57 BL/6小鼠对rDer f 2有较好的免疫应答，佐剂以百日咳毒素弗氏佐剂为佳。
Der f 2 is the group 2 major allergen of a house dust mite (Dermatophagoides farinae) and its function has been recently suggested. To determine the optimal condition of sensitization to recombinant Der f 2 (rDer f 2) in murine model of asthma, we compared the effectiveness with different adjuvants in BALB/c and C57BL/6 mice. Mice from both strains sensitized with rDer f 2 by intraperitoneal injection or subcutaneous injection on days 1 and 14. The dosage was 20 µg. Freund's adjuvants with pertussis toxin (FP) or alum alone were used as adjuvants. On days 28, 29, and 30, mice were challenged intranasally with 0.1% rDer f 2. We evaluated airway hyperresponsivenss, eosinophil proportion in lung lavage, airway inflammation, and serum allergen specific antibody responses. Naive mice were used as controls. Airway hyperresponsiveness was increased in C57BL/6 with FP, and BALB/c with alum (PC200: 13.5±6.3, 13.2±6.7 vs. >50 mg/ml, p<0.05). The eosinophil proportion was increased in all groups; C57BL/6 with FP, BALB/c with FP, C57BL/6 with alum, BALB/c with alum (24.8±3.6, 20.3±10.3, 11.0±6.9, 5.7±2.8, vs. 0.0±0.0%, p<0.05). The serum allergen specific IgE levels were increased in C57BL/6 with FP or alum (OD: 0.8±1.4, 1.1±0.8, vs. 0.0±0.0). C57BL/6 mice were better responders to rDer f 2 and as for adjuvants, Freund's adjuvant with pertussis toxin was better.