A single-step preparation of carbohydrate functionalized monoliths for separation and trapping of polar compounds.
A single-step preparation of carbohydrate functionalized monoliths for separation and trapping of polar compounds.
复制标题
DOI：
10.1016/j.chroma.2020.461481
复制
发表时间：
2020-08
期刊：
影响因子：
--
通讯作者：
Jincai Wang;Jialiang Guo;Haowen Chen;Xiaoling Huang;G. Somsen;F. Song;Zhengjin Jiang
中科院分区：
文献类型：
--
作者：
Jincai Wang;Jialiang Guo;Haowen Chen;Xiaoling Huang;G. Somsen;F. Song;Zhengjin Jiang
本文提出了一种利用点击反应制备碳水化合物（葡萄糖和麦芽糖）功能化整体材料的一步共聚策略。首先，通过Cu（I）催化的末端炔与碳水化合物衍生物的叠氮化物的1，3-偶极环加成反应（炔-叠氮化物反应），合成了新型的碳水化合物官能化的甲基丙烯酸酯单体。然后通过单步原位聚合制备相应的碳水化合物功能化整体柱。采用扫描电子显微镜、傅立叶变换红外光谱和纳米液相色谱对制备的整体柱的理化性质和性能进行了评价。优化后的整体柱对核苷、酚类化合物和苯甲酸衍生物等极性化合物具有良好的分离性能。该整体柱也非常适用于从人IgG胰蛋白酶消化物中选择性和有效地富集糖肽。本研究不仅为极性化合物的分离和选择性捕集提供了一种新型的亲水性柱，而且为制备碳水化合物功能化整体柱提供了一种简便有效的方法。
A single-step copolymerization strategy was developed for the preparation of carbohydrate (glucose and maltose) functionalized monoliths using click reaction. Firstly, novel carbohydrate-functionalized methacrylate monomers were synthesized through Cu(I)-catalyzed 1,3-dipolar cycloaddition (alkyne-azide reaction) of terminal alkyne with azide of carbohydrate derivatives. The corresponding carbohydrate functionalized monolithic columns were then prepared through a single-stepin-situcopolymerization. The physicochemical properties and performance of the fabricated monolithic columns were evaluated using scanning electron microscopy, Fourier-transform infrared spectroscopy, and nano-liquid chromatography. For the optimized monolithic column, satisfactory column permeability and good separation performance were demonstrated for polar compounds including nucleoside, phenolic compounds and benzoic acid derivatives. The monolithic column is also highly useful for selective and efficient enrichment of glycopeptides from human IgG tryptic digests. This study not only provided a novel hydrophilic column for separation and selective trapping of polar compounds, but also proposed a facile and efficient approach for preparing carbohydrate functionalized monoliths.