Methods special issue: RNA-targeting technologies.
Methods special issue: RNA-targeting technologies.
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方法特刊：RNA 靶向技术。
DOI：
10.1016/j.ymeth.2023.02.006
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
O'Connell,MitchellR
中科院分区：
文献类型：
--
作者：
Knott,GavinJ;Lapinaite,Audrone;O'Connell,MitchellR
RNA是所有生物学的核心。RNA作为治疗和治疗靶点的日益突出，促使研究人员对RNA的功能提出越来越具有挑战性的问题，从序列到修饰，定位，形状和动力学。解决这些问题需要工具来特异性地识别RNA并在体外和体内研究其功能。虽然有一个诱惑，拥抱一个单一的平台技术，允许强大的RNA靶向，瞬时性，结构复杂性，和动态的RNA使其固有的难以靶向。为了实现RNA的潜力并研究其功能复杂性的广度，需要一个丰富的工具箱，其中包含经过验证并针对感兴趣的目标量身定制的复杂解决方案。本方法特刊的目的是提供当前精确靶向RNA工具包的一些成员的快照。从小分子到更大的可编程大分子复合物，这篇文章的汇编描述了一个新兴的不同工具的领域，这些工具是解决RNA及其应用复杂性的重要步骤。RNA是一种高度动态和适应性强的分子，可以根据其细胞定位，相互作用伙伴和代谢细胞状态呈现各种复杂和独特的构象。这种改变其形状的能力使RNA能够在细胞中执行广泛的基本功能。某些类型的RNA的结构性质使其成为有吸引力的治疗靶点，特别是如果它编码或调节不可药用的蛋白质。靶向RNA的方法之一依赖于RNA和小有机分子（称为配体）之间的选择性相互作用。了解靶向和脱靶RNA-配体相互作用对于新药和疗法的开发至关重要。Sexton等人[1]通过开发RNA连接测序的光亲和性评价（PEARL-seq）方法，解决了在体外和生理相关条件下检测全转录组RNA-配体相互作用的需求。这种方法是基于通过紫外线照射将感兴趣的配体（其具有连接的光反应性基团）交联到近端RNA分子。然后，高通量测序允许交联位点（即RNA-配体相互作用位点）的单核苷酸分辨率检测。配体何时、何地以及如何与结构化RNA相互作用的知识为识别RNA中的新药物位点铺平了道路。
RNA is the centerpiece for all biology. The rising prominence of RNA as a therapeutic and therapeutic target has driven researchers to ask increasingly challenging questions about the function of RNA from sequence to modifications, localization, shape, and dynamics. Answering these questions requires tools to specifically recognize RNA and investigate its function both in vitro and in vivo. While there is a temptation to embrace a single platform technology that allows for robust RNA-targeting, the transience, structural complexity, and dynamics of RNA make it inherently difficult to target. To realize the potential of RNA and study the breadth of its functional complexity requires a rich toolbox of sophisticated solutions that are validated and tailored to a target of interest. The goal behind this Methods Special Issue is to provide a snapshot of some members of the current toolkit for precisely targeting RNA. From small molecules to larger programmable macromolecular complexes, this compilation of articles describes an emerging field of diverse tools that are significant steps towards tackling the complexity of RNA and its applications.RNA is a highly dynamic and adaptable molecule that can assume a variety of complex and distinct conformations, depending on its cellular localization, interaction partners, and metabolic cell state. This ability to change its shape allows RNA to perform a wide range of essential functions in cells. The structured nature of some classes of RNA makes it an attractive therapeutic target, especially if it encodes or regulates an undruggable protein. One of the approaches to target RNA relays on selective interactions between the RNA and small organic molecules, known as ligands. Understanding the on-and off-target RNA-ligand interactions is critical for the development of new drugs and therapies. Sexton et al.[1] address the need to detect transcriptome-wide RNA-ligand interactions in vitro and in physiologically relevant conditions by developing the Photoaffinity Evaluation of RNA Ligation-Sequencing (PEARL-seq) approach. This approach is based on crosslinking the ligand of interest (that has a photoreactive group attached) to the proximal RNA molecules via UV-irradiation. High throughput sequencing then allows single nucleotide resolution detection of the cross-linked site, that is, the RNA-ligand interaction site. Knowledge of when, where, and how ligands interact with structured RNA paves the way to identifying novel druggable sites in RNA.
使用光亲和平台 PEARL-seq 测定 RNA-配体相互作用。
影响因子：
4.8
作者：
A. N. Sexton;Lee E. Vandivier;Jennifer C Petter;Herschel Mukherjee;J. Craig Blain
通讯作者：
J. Craig Blain
RNA 的序列特异性靶向。
影响因子：
4.8
作者：
Lukas Rösner;Dennis Reichert;Kristina Rau;N. Muthmann;A. Rentmeister
通讯作者：
A. Rentmeister
影响因子：
4.8
作者：
Burris, Brandon Joseph Davis;Vargas, Adrian Moises Molina;Park, Brandon J.;O'Connell, Mitchell R.
通讯作者：
O'Connell, Mitchell R.