Synthesis of tumor-associated MUC1-glycopeptides and their multivalent presentation by functionalized gold colloids.
Synthesis of tumor-associated MUC1-glycopeptides and their multivalent presentation by functionalized gold colloids.
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DOI：
10.1039/c4ob01339e
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发表时间：
2015-01
影响因子：
3.2
通讯作者：
Isabella Tavernaro;S. Hartmann;L. Sommer;H. Hausmann;Christian Rohner;M. Ruehl;A. Hoffmann-Roeder;S. Schlecht
中科院分区：
文献类型：
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作者：
Isabella Tavernaro;S. Hartmann;L. Sommer;H. Hausmann;Christian Rohner;M. Ruehl;A. Hoffmann-Roeder;S. Schlecht
粘蛋白MUC 1是一种参与基本生物学过程的糖蛋白，可以发现其在上皮肿瘤细胞上过度表达并具有明显改变的聚糖模式;因此，它是寻求有效的基于碳水化合物的癌症疫苗和免疫治疗剂的有希望的靶结构。天然糖肽抗原仅表明低免疫原性和T细胞非依赖性免疫应答;然而，该主要缺点可通过将糖肽抗原多价偶联至免疫刺激载体平台来克服。特别是，金纳米颗粒非常适合作为模板的糖肽抗原的多价介绍，由于其显着高的表面与体积比结合其高的生物稳定性。在这项工作中，新的MUC 1-糖肽抗原的合成及其耦合到不同大小的金纳米粒子。此外，还介绍了一种新的检测抗原抗体结合的斑点免疫分析方法。
The mucin MUC1 is a glycoprotein involved in fundamental biological processes, which can be found over-expressed and with a distinctly altered glycan pattern on epithelial tumor cells; thus it is a promising target structure in the quest for effective carbohydrate-based cancer vaccines and immunotherapeutics. Natural glycopeptide antigens indicate only a low immunogenicity and a T-cell independent immune response; however, this major drawback can be overcome by coupling of glycopeptide antigens multivalently to immunostimulating carrier platforms. In particular, gold nanoparticles are well suited as templates for the multivalent presentation of glycopeptide antigens, due to their remarkably high surface-to-volume ratio in combination with their high biostability. In this work the synthesis of novel MUC1-glycopeptide antigens and their coupling to gold nanoparticles of different sizes are presented. In addition, the development of a new dot-blot immunoassay to test the potential antigen-antibody binding is introduced.