Biological Control of Cyclamen Gray Mould (Botrytis cinerea) by Serratia marcescens B2
Biological Control of Cyclamen Gray Mould (Botrytis cinerea) by Serratia marcescens B2
复制标题
粘质沙雷氏菌 B2 对仙客来灰霉病 (Botrytis cinerea) 的生物防治
DOI：
10.3186/jjphytopath.62.559
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
K. Akutsu
中科院分区：
文献类型：
--
作者：
H. Iyozumi;T. Komagata;K. Hirayae;K. Tsuchiya;T. Hibi;K. Akutsu
以高度分解几丁质的粘质沙雷菌B2菌株处理仙客来花瓣，24小时后用灰葡萄孢分生菌攻毒接种。该菌可抑制温室内仙客来贴附花瓣上真菌病的发病率约60%。菌株B2对苯并咪唑和二甲氧基肟类杀菌剂均有抗性，其对灰霉菌的防治效果与200ppm二甲氧基肟类杀菌剂异丙二酮相当。虽然分离物B2在初次施用后两周内未能在花瓣和叶片上存活，但放置在仙客来植物基部附近土壤上的叶盘上的种群数量在此期间增加了10倍，因为灰霉经常发生。在施用分离物B2后1小时、3天或7天，用灰孢杆菌攻毒接种仙客来叶片，然后放置在温室植株基部附近。真菌孢子的形成被抑制85%以上。粘质葡萄球菌B2对椎间盘真菌产孢的抑制效果明显优于异丙酮。
Cyclamen petals were treated with a highly chitinolytic bacterium, Serratia marcescens isolate B2, and 24hr later, were challenge-inoculated with Botrytis cinerea conidia. The bacterium suppressed fungal disease incidence by ca. 60% on attached petals of cyclamen in the greenhouse. The efficacy of isolate B2 against gray mould caused by B. cinerea, which was resistant to benzimidazole and dicarboximide fungicides, was nearly equal to that of 200ppm of iprodione, a dicarboximide fungicide. Although isolate B2 failed to survive on petals and leaves more than two weeks after the initial application, populations on leaf discs placed on the soil near the base of the cyclamen plant, where gray mould regularly developed, increased 10-fold during this period. Cyclamen leaf discs were challenge-inoculated with B. cinerea conidia either 1hr, 3 days or 7 days after isolate B2 application, then placed near the base of the plant in the greenhouse. Fungal sporulation on the discs was suppressed by more than 85%. S. marcescens B2 was significantly more effective than iprodione treatment against fungal sporulation on the discs.