Categorizing Cells on the Basis of their Chemical Profiles: Progress in Single-Cell Mass Spectrometry.
Categorizing Cells on the Basis of their Chemical Profiles: Progress in Single-Cell Mass Spectrometry.
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DOI：
10.1021/jacs.6b12822
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发表时间：
2017-03-22
影响因子：
15
通讯作者：
Sweedler JV
中科院分区：
文献类型：
--
作者：
Comi TJ;Do TD;Rubakhin SS;Sweedler JV
大细胞群中单个细胞之间的化学差异提供了有关生物体稳态和疾病状态发展的独特信息。即使基因相同的细胞谱系也会因局部微环境和随机过程而出现分歧。细胞中某些成分的微小样本量和低丰度阻碍了我们对细胞异质性的理解。尽管扩增方法促进了单细胞基因组学和转录组学的发展，但由于缺乏有效的扩增方法以及细胞成分的广泛多样性，代谢物和蛋白质的表征仍然具有挑战性。质谱分析凭借其精湛的灵敏度、大的动态范围以及表征数百至数千种化合物的能力，已成为研究个体细胞代谢物谱的支持技术。虽然仪器的进步提高了品质因数，但以高通量获取测量结果和从大量细胞中采样仍然不是常规的。在本视角中，我们重点介绍基于质谱的单细胞分析的当前趋势和进展，重点关注支持下一代单细胞测量的技术。
The chemical differences between individual cells within large cellular populations provide unique information on organisms’ homeostasis and the development of diseased states. Even genetically identical cell lineages diverge due to local microenvironments and stochastic processes. The minute sample volumes and low abundance of some constituents in cells hinder our understanding of cellular heterogeneity. Although amplification methods facilitate single-cell genomics and transcriptomics, the characterization of metabolites and proteins remains challenging both because of the lack of effective amplification approaches and the wide diversity in cellular constituents. Mass spectrometry has become an enabling technology for the investigation of individual cellular metabolite profiles with its exquisite sensitivity, large dynamic range, and ability to characterize hundreds to thousands of compounds. While advances in instrumentation have improved figures of merit, acquiring measurements at high throughput and sampling from large populations of cells are still not routine. In this Perspective, we highlight the current trends and progress in mass-spectrometry-based analysis of single cells, with a focus on the technologies that will enable the next generation of single-cell measurements.