Enterococcus faecalis plasmid pAD1-encoded Fst toxin affects membrane permeability and alters cellular responses to lantibiotics.
Enterococcus faecalis plasmid pAD1-encoded Fst toxin affects membrane permeability and alters cellular responses to lantibiotics.
复制标题
粪肠球菌质粒 pAD1 编码的 Fst 毒素影响膜通透性并改变细胞对羊毛硫抗生素的反应。
DOI：
10.1128/jb.185.7.2169-2177.2003
复制
发表时间：
2003
影响因子：
3.2
通讯作者：
Ehli,ErikA
中科院分区：
文献类型：
--
作者：
Weaver,KeithE;Weaver,DarielM;Wells,CarolL;Waters,ChristopherM;Gardner,MarshallE;Ehli,ErikA
Fst是由粪肠球菌质粒pAD 1的partoxin-antitoxin stability determinator编码的肽毒素。Fst的细胞内过量产生导致所有细胞大分子合成的同时抑制伴随着细胞生长抑制和损害细胞膜的完整性。细胞没有溶解或明显泄漏细胞内内容物，但在染色体分配和细胞分裂中有特定的缺陷。细胞外添加合成Fst对细胞生长没有影响。自发性抗Fst突变体的表型与膜组成的变化一致。有趣的是，过量生产的Fst敏感细胞的lantibiotic乳酸链球菌素，和Fst抗性突变体的交叉耐药乳酸链球菌素和pAD 1编码的溶细胞素。
Fst is a peptide toxin encoded by thepartoxin-antitoxin stability determinant ofEnterococcus faecalisplasmid pAD1. Intracellular overproduction of Fst resulted in simultaneous inhibition of all cellular macromolecular synthesis concomitant with cell growth inhibition and compromised the integrity of the cell membrane. Cells did not lyse or noticeably leak intracellular contents but had specific defects in chromosome partitioning and cell division. Extracellular addition of synthetic Fst had no effect on cell growth. Spontaneous Fst-resistant mutants had a phenotype consistent with changes in membrane composition. Interestingly, overproduction of Fst sensitized cells to the lantibiotic nisin, and Fst-resistant mutants were cross-resistant to nisin and the pAD1-encoded cytolysin.