Isolation of human cytomegalovirus from peripheral blood T cells of renal transplant patients.
Isolation of human cytomegalovirus from peripheral blood T cells of renal transplant patients.
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从肾移植患者外周血 T 细胞中分离人巨细胞病毒。
DOI：
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发表时间：
1982
期刊：
影响因子：
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通讯作者：
Garnett Hm
中科院分区：
文献类型：
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作者：
Garnett Hm
先前的报道已经证明，人CMV可以从从排出CMV的患者的外周血的单核白细胞部分中分离。许多肾移植患者表现出活动性CMV感染的证据，并且在这些患者中T细胞应答通常受损。因此，本研究的目的是确定是否可以从肾移植后3个月的病毒尿患者的T和/或B淋巴细胞中分离出病毒。从小百分比的T细胞（3/10（6）个细胞）中回收病毒，但不能从B细胞中回收病毒。因此，似乎T细胞的病毒感染不能解释在这些移植患者中观察到的细胞介导的免疫抑制，除非T细胞的特定调节亚群被感染。然而，从T细胞中分离出CMV意味着它们必须被认为是体内病毒持续存在的可能细胞之一。
Previous reports have demonstrated that human CMV can be isolated from the mononuclear leukocyte fraction of peripheral blood taken from patients excreting CMV. Many renal transplant patients show evidence of an active CMV infection, and in these patients T cell responses are often impaired. Thus the purpose of the current study was to determine whether the virus could be isolated from either the T and/or B lymphocytes of viruric patients 3 months after renal transplantation. The virus was recovered from a small percentage of T cells (3 per 10(6) cells), but not from B cells. Thus it would appear that virus infection of T cells cannot explain the depressed cell-mediated immunity observed in these transplant patients unless a specific regulatory subset of T cells is infected. However, the isolation of CMV from T cells implies that they must be considered as one of the possible cells for virus persistence in vivo.