CHEMICAL AND PHYSICOCHEMICAL CHARACTERIZATION OF FIBERS FROM LAMINARIA-DIGITATA (KOMBU BRETON) - A PHYSIOLOGICAL APPROACH
CHEMICAL AND PHYSICOCHEMICAL CHARACTERIZATION OF FIBERS FROM LAMINARIA-DIGITATA (KOMBU BRETON) - A PHYSIOLOGICAL APPROACH
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DOI：
10.1002/jsfa.2740550307
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发表时间：
1991-01-01
影响因子：
4.1
通讯作者：
LAHAYE, M
中科院分区：
文献类型：
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作者：
FLEURY, N;LAHAYE, M
测定了棕色海水海带（Laminaria digitata，kombu布雷东）可溶性和不溶性膳食纤维的含量和化学组成。 使用两种酶重量法测定（1）根据AOAC程序的修改的可溶性和不溶性纤维的含量，和（2）分别用于模拟消化的胃和肠阶段的37 ℃和pH 2.0和7.5的盐水缓冲液中可溶性纤维的分布。 两种方法测得的总膳食纤维含量相似（37.3%和40.0%），其中84.8%~ 87.4%为可溶性。 可溶性纤维的分配可能发生在消化过程中，因为在pH 2.0的盐水缓冲液中回收了49.3%，而在pH 7.5的盐水缓冲液中回收了50.7%。 褐藻膳食纤维的溶解性与褐藻多糖的化学性质有关。 岩藻聚糖和laminarans基本上是可溶的pH值为2.0和藻酸盐在pH值为7.5，和不溶性纤维主要由cellulose.Oil吸附和水化性能（吸收，保留和溶胀）在水中，154 mM NaCl，和38 mM CaCl 2在20和37 ℃的三个颗粒大小的L digitata进行了测量。 吸油量较低（0.16-0.41 g g ~（-1）），且与纤维粒径有关。 水化性能更重要的小颗粒，除了在氯化钙溶液中，并遵循顺序水> NaCl >氯化钙。 37 ℃时的吸水率和溶胀率大于20 ℃时的吸水率和溶胀率。 用离子强度约0.15的溶液观察到的水合性质的总体降低被解释为反映了带电多糖之间的静电排斥的降低。 最低的吸水率，保水性和膨胀得到与氯化钙的解决方案，并与已知的选择性亲和力的海藻酸钙。因此，L digitata是特别丰富的可溶性膳食纤维，这些具有物理化学特性的多糖存在。 水的吸收、吸收和膨胀可以根据各种物理和化学参数进行调节。
The content and chemical composition of soluble and insoluble dietary fibres from the brown marine alga Laminaria digitata (kombu breton) were determined. Two enzymic-gravimetric methods were used to determine (1) the content of soluble and insoluble fibres according to a modification of the AOAC procedure, and (2) the distribution of the soluble fibres in saline buffer at 37-degrees-C and at pH 2.0 and 7.5 used to simulate the gastric and intestinal phases of digestion, respectively. The total dietary fibre contents obtained by the two methods were similar (37.3 and 40.0%) and of these 84.8-87.4% was soluble. A partitioning of soluble fibres may occur during digestion since 49.3% was recovered in saline buffer at pH 2.0 whereas 50.7% was recovered in saline buffer at pH 7.5. Solubility of dietary fibres was related to the chemistry of brown algal polysaccharides. Fucans and laminarans were essentially soluble at pH 2.0 and alginate at pH 7.5, and insoluble fibres consisted primarily of cellulose.Oil adsorption and hydration properties (uptake, retention and swelling) in water, 154 mM NaCl, and 38 mM CaCl2 at 20 and 37-degrees-C of three particle sizes of L digitata were measured. Oil adsorptiion was low (0.16-0.41 g g-1) and was related to the particle size of the fibres. Hydration properties were more important with small particles except in CaCl2 solution and followed the order water > NaCl > CaCl2. Water uptake and swelling were greater at 37-degrees-C than at 20-degrees-C. The overall decrease in hydration properties observed with solutions of ionic strength approximately 0.15 was interpreted as reflecting the decrease in the electrostatic repulsion between charged polysaccharides. The lowest water uptake, water retention and swelling were obtained with solutions of CaCl2 and were related to the known selective affinity of alginate for calcium.Thus, L digitata is especially rich in soluble dietary fibres, and these have physico-chemical properties characteristic of the polysaccharides present. Water absorption and uptake and welling can be modulated according to various physical and chemical parameters.