Lipid composition greatly affects the in vitro surface activity of lung surfactant protein mimics.
Lipid composition greatly affects the in vitro surface activity of lung surfactant protein mimics.
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脂质组成极大地影响肺表面活性蛋白模拟物的体外表面活性。
DOI：
10.1016/j.colsurfb.2007.01.001
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Barron,AnneliseE
中科院分区：
文献类型：
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作者：
Seurynck-Servoss,ShannonL;Brown,NathanJ;Dohm,MichelleT;Wu,CindyW;Barron,AnneliseE
开发功能性仿生肺表面活性剂（LS）替代品的一个关键方面是选择合成脂质混合物和表面活性剂蛋白（SPs）或其合适的模拟物。研究阐明了不同脂质和表面活性剂蛋白在天然LS中的作用，为开发具有与天然材料相当性能的合成LS替代品提供了必要的关键信息。在这项研究中，研究了基于肽和肽的肺表面活性蛋白模拟物SP-B和SP-C的体外表面活性行为，使用三种不同的脂质配方。这些脂质混合物是从常用的SP模拟物的测试和表征中选择的：(1)双棕榈酰磷脂酰胆碱：棕榈酰磷脂酰甘油7:3 (w/w) (PCPG),(2)双棕榈酰磷脂酰胆碱：棕榈酰磷脂酰甘油：棕榈酸68:22:9 (w/w) (TL)，(3)双棕榈酰磷脂酰胆碱：棕榈酰磷脂酰胆碱：棕榈酰磷脂酰甘油：棕榈酰磷脂酰乙醇胺：棕榈酰磷脂酰丝氨酸：胆固醇16:10:3:1:3:2 (w/w) （IL）。脂质混合物和脂质/肽或脂质/类肽制剂在体外使用Langmuir-Wilhelmy表面平衡、表面膜形态的荧光显微成像和脉动气泡表面计进行表征。结果表明，在存在和不存在SP模拟物的情况下，三种脂质制剂表现出显著不同的表面活性行为，其中TL制剂的体外仿生行为最好。具体来说，根据动态PBS和LWSB研究，TL配方能够在生理温度下达到低表面张力，动态PBS研究表明，与天然LS类似，这种情况发生在最小的压缩量下。
A crucial aspect of developing a functional, biomimetic lung surfactant (LS) replacement is the selection of the synthetic lipid mixture and surfactant proteins (SPs) or suitable mimics thereof. Studies elucidating the roles of different lipids and surfactant proteins in natural LS have provided critical information necessary for the development of synthetic LS replacements that offer performance comparable to the natural material. In this study, the in vitro surface-active behaviors of peptide- and peptoid-based mimics of the lung surfactant proteins, SP-B and SP-C, were investigated using three different lipid formulations. The lipid mixtures were chosen from among those commonly used for the testing and characterization of SP mimics—(1) dipalmitoyl phosphatidylcholine:palmitoyloleoyl phosphatidylglycerol 7:3 (w/w) (PCPG), (2) dipalmitoyl phosphatidylcholine:palmitoyloleoyl phosphatidylglycerol:palmitic acid 68:22:9 (w/w) (TL), and (3) dipalmitoyl phosphatidylcholine:palmitoyloleoyl phosphatidylcholine:palmitoyloleoyl phosphatidylglycerol:palmitoyloleoyl phosphatidylethanolamine:palmitoyloleoyl phosphatidylserine:cholesterol 16:10:3:1:3:2 (w/w) (IL). The lipid mixtures and lipid/peptide or lipid/peptoid formulations were characterized in vitro using a Langmuir–Wilhelmy surface balance, fluorescent microscopic imaging of surface film morphology, and a pulsating bubble surfactometer. Results show that the three lipid formulations exhibit significantly different surface-active behaviors, both in the presence and absence of SP mimics, with desirable in vitro biomimetic behaviors being greatest for the TL formulation. Specifically, the TL formulation is able to reach low-surface tensions at physiological temperature as determined by dynamic PBS and LWSB studies, and dynamic PBS studies show this to occur with a minimal amount of compression, similar to natural LS.