NUCLEOTIDE-SEQUENCES OF 2 MESSENGER-RNAS FOR RAT-BRAIN MYELIN PROTEOLIPID PROTEIN
NUCLEOTIDE-SEQUENCES OF 2 MESSENGER-RNAS FOR RAT-BRAIN MYELIN PROTEOLIPID PROTEIN
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DOI：
10.1016/0092-8674(85)90289-2
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发表时间：
1985-01-01
期刊：
影响因子：
64.5
通讯作者：
SUTCLIFFE, JG
中科院分区：
文献类型：
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作者：
MILNER, RJ;LAI, C;SUTCLIFFE, JG
编码大鼠脑蛋白脂蛋白（PLP）（中枢神经系统髓磷脂的主要蛋白质成分）的3200和1600个核苷酸mrna在它们的5”末端是异质的，在它们的3”聚腺苷化位点上是不同的，并且是由一个基因转录的。这些mrna在出生后首次出现，编码277个相同的氨基酸蛋白，与牛蛋白序列99%相同。因此，在哺乳动物的进化过程中，PLP是高度保守的。一个单氨基端蛋氨酸在翻译后被移除，表明PLP不需要信号肽序列插入髓鞘膜。小鼠和猴子利用3200个而不是1600个核苷酸mRNA，这表明两种大小的大鼠PLP mRNA在功能上没有必要。
The 3200 and 1600 nucleotide mRNAs encoding rat brain proteolipid protein (PLP), the major protein component of central nervous system myelin, are heterogeneous at their 5'' ends, differ in their 3'' polyadenylation sites, and are transcribed from a single gene. The mRNAs, which first appear postnatally, encode identical 277 amino acid proteins that are 99% identical to the bovine protein sequence. Thus, PLP has been highly conserved during mammalian evolution. A single amino-terminal methionine is removed post-translationally, indicating that PLP does not require a signal peptide sequence for insertion into the myelin membrane. Mouse and monkey utilize the 3200 but not the 1600 nucleotide mRNA, suggesting that there is no functional necessity for two sizes of rat PLP mRNAs.