Biomedical analysis of rat body hair after hindlimb suspension for 14 days
Biomedical analysis of rat body hair after hindlimb suspension for 14 days
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DOI：
10.1016/j.actaastro.2011.12.016
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发表时间：
2012-04
影响因子：
3.5
通讯作者：
M. Terada;F. Kawano;N. Ishioka;A. Higashibata;H. Majima;Takashi Yamazaki;T. Watanabe-Asaka;M. Niihori;Reiko Nakao;Shin Yamada;C. Mukai;Y. Ohira
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文献类型：
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作者：
M. Terada;F. Kawano;N. Ishioka;A. Higashibata;H. Majima;Takashi Yamazaki;T. Watanabe-Asaka;M. Niihori;Reiko Nakao;Shin Yamada;C. Mukai;Y. Ohira
对对照大鼠和后肢悬浮 Wistar Hannover 大鼠（每只 n=5）分析了大鼠体毛中 26 种矿物质的水平。我们量化了本实验中 22 种矿物质的含量。然而，我们无法定量测量 4 种矿物质（Be、V、Cd 和 Hg）的水平，因为它们低于检测限。在 22 种量化的矿物质中，19 种矿物质的水平在对照组和后肢悬挂组之间没有显着差异。后肢悬挂组中 3 种矿物质（Pb、Cr 和 Al）的水平往往高于对照组；然而，这种差异并不显着。两组之间其他 3 种矿物质（I、K 和 Mg）的浓度存在显着差异。后肢悬吊组碘（I）水平比对照组高58.2%（P<0.05）。实验组中钾 (K) 和镁 (Mg) 水平分别降低了 55.2% 和 20.4%（两种情况下 P<0.05）。这些结果表明，由于身体或精神压力（例如后肢悬垂）引起的矿物质代谢的生理变化反映在体毛中。日本宇宙航空研究开发机构（JAXA）启动了一项人体研究，通过分析在国际空间站（ISS）停留6个月的宇航员的头发样本，调查长期太空飞行对基因表达和矿物质代谢的影响。我们认为，后肢悬挂14天可以模拟极其恶劣的环境（例如太空飞行）的影响，因为后肢悬挂模型即使在短期内也会引起骨骼肌、骨骼和体液转移的快速生理变化。这些结果还表明，我们可以通过分析人类头皮毛干来检测对身体的各种影响。
The levels of 26 minerals in rat body hair were analyzed in control and hindlimb-suspended Wistar Hannover rats (n=5 each). We quantified the levels of 22 minerals in this experiment. However, we were unable to measure the levels of 4 minerals (Be, V, Cd, and Hg) quantitatively because they were below the limit of detection. Of the 22 quantified, the levels of 19 minerals were not significantly different between control and hindlimb-suspended groups. The levels of 3 minerals (Pb, Cr, and Al) tended to be higher in the hindlimb-suspended group than in the control group; however, this difference was not significant. The concentrations of 3 other minerals (I, K, and Mg) were significantly different between the 2 groups. The iodine (I) level was 58.2% higher in the hindlimb-suspended group than in the control group (P<0.05). Potassium (K) and magnesium (Mg) levels were 55.2% and 20.4% lower, respectively, in the experimental group (P<0.05 in both cases). These results indicate that a physiological change in mineral metabolism resulting from physical or mental stress, such as hindlimb suspension, is reflected in body hair. The Japan Aerospace Exploration Agency (JAXA) has initiated a human research study to investigate the effects of long-term space flight on gene expression and mineral metabolism by analyzing hair samples of astronauts who stayed in the International Space Station (ISS) for 6 months. We believe that hindlimb suspension for 14 days can simulate the effects of an extremely severe environment, such as space flight, because the hindlimb suspension model elicits a rapid physiological change in skeletal muscle, bone, and fluid shift even in the short term. These results also suggest that we can detect various effects on the body by analyzing the human scalp hair shaft.