Complete genome sequences of two highly divergent Japanese isolates of Plantago asiatica mosaic virus
Complete genome sequences of two highly divergent Japanese isolates of Plantago asiatica mosaic virus
复制标题
车前子花叶病毒两种高度分化的日本分离株的完整基因组序列
DOI：
10.1007/s00705-016-3110-6
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发表时间：
2016
影响因子：
2.7
通讯作者：
S. Fuji
中科院分区：
文献类型：
--
作者：
K. Komatsu;K. Yamashita;K. Sugawara;M. Verbeek;N. Fujita;K. Hanada;T. Uehara;S. Fuji
车前子花叶病毒（PlAMV）是马铃薯病毒属的成员，具有极其广泛的宿主范围。 对百合造成严重损害。在这里，我们报道了两种新的日本 PlAMV 分离株的完整核苷酸序列，一种来自真双子叶植物紫花地丁 (Viola grypoceras) (PlAMV-Vi)，另一种来自真双子叶植物灌木南天竹 (Nandina DomesticaThunb)。 （PLAMV-新泽西州）。它们的基因组包含五个开放阅读框（ORF），这是马铃薯病毒的特征。令人惊讶的是，这些分离株彼此之间以及与其他 PlAMV 分离株（包括日本百合和美国南天竹的分离株）仅显示 76.0–78.0% 的序列同一性。 ORF1 编码的复制酶编码区的氨基酸比对表明，甲基转移酶和解旋酶结构域之间的区域比其他区域不太保守，有多个插入和/或缺失。全长核苷酸序列的系统发育分析揭示了 PlAMV 分离株的系统发育聚类与原始寄主植物之间存在中等相关性。这项研究揭示了日本存在两种高度不同的 PlaAMV 分离株。
Plantago asiatica mosaic virus (PlAMV) is a member of the genusPotexvirusand has an exceptionally wide host range. It causes severe damage to lilies. Here we report on the complete nucleotide sequences of two new Japanese PlAMV isolates, one from the eudicot weedViola grypoceras(PlAMV-Vi), and the other from the eudicot shrubNandina domesticaThunb. (PlAMV-NJ). Their genomes contain five open reading frames (ORFs), which is characteristic of potexviruses. Surprisingly, the isolates showed only 76.0–78.0 % sequence identity with each other and with other PlAMV isolates, including isolates from Japanese lily and American nandina. Amino acid alignments of the replicase coding region encoded by ORF1 showed that the regions between the methyltransferase and helicase domains were less conserved than other regions, with several insertions and/or deletions. Phylogenetic analyses of the full-length nucleotide sequences revealed a moderate correlation between phylogenetic clustering and the original host plants of the PlAMV isolates. This study revealed the presence of two highly divergent PlAMV isolates in Japan.
郁金香病毒 X (TVX-J) 的完整核苷酸序列：Potexvirus 属内物种和毒株之间的边界。
DOI：
--
发表时间：
2001
期刊：
Arch.Virol. 146
影响因子：
--
作者：
Yamaji;Y.;Kagiwada;S.;Nakabayashi;H.;Ugaki;M.;Namba;S.
通讯作者：
S.