Absence of Borrelia burgdorferi DNA in cutaneous B-cell lymphomas from the United States.
Absence of Borrelia burgdorferi DNA in cutaneous B-cell lymphomas from the United States.
复制标题
来自美国的皮肤 B 细胞淋巴瘤中不存在伯氏疏螺旋体 DNA。
DOI：
10.1034/j.1600-0560.2001.281002.x
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发表时间：
2001
影响因子：
1.7
通讯作者：
Cerroni,L
中科院分区：
文献类型：
--
作者：
Wood,GS;Kamath,NV;Guitart,J;Heald,P;Kohler,S;Smoller,BR;Cerroni,L
Background:An association betweenBorrelia burgdorferiand cutaneous B‐cell lymphoma (CBCL) has been made in several European countries. The evidence in favor of such an association has recently been based on more definitive tests for the pathogenetic role ofB. burgdorferiin CBCL, including positive cultures or polymerase chain reaction (PCR) amplification of borrelial DNA from lesional skin. However, there is only one report ofB. burgdorferiin four North American cases of B‐cell lymphoma.Methods:We retrieved 38 cases of primary and secondary CBCL from different geographic regions of the United States. Two separate techniques were used to detect borrelial DNA by PCR, a nested PCR method to amplify aB. burgdorferi‐specific gene as well as a borrelial chromosomal Ly‐1 clone amplification method. Southern blot hybridization was used for confirmation of the PCR results.Results:NoB. burgdorferi‐specific DNA was detected in any of the 38 CBCL cases, whereas detectable PCR products were obtained with our positive controls.Conclusions:Our findings, in light of previous studies, suggest thatB. burgdorferiplays a minimal role in the development or pathogenesis of CBCL in the United States. The findings also suggest that the geographic variations in the clinical manifestations ofB. burgdorferiare indeed real and may be secondary to the genetic and phenotypic differences betweenB. burgdorferistrains present in Europe and North America.
