Gene-Z: a device for point of care genetic testing using a smartphone
Gene-Z: a device for point of care genetic testing using a smartphone
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DOI：
10.1039/c2lc21226a
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发表时间：
2012-01-01
期刊：
影响因子：
6.1
通讯作者：
Hashsham, Syed A.
中科院分区：
文献类型：
--
作者：
Stedtfeld, Robert D.;Tourlousse, Dieter M.;Hashsham, Syed A.
到2012年，护理点（POC）检测将占590亿美元体外诊断市场的三分之一左右。进行多重基因检测和无线连接的能力正在成为未来POC设备的关键属性。在这项研究中，一个廉价的，用户友好的和紧凑的设备（称为Gene-Z）的快速定量检测多个遗传标记具有高灵敏度和特异性。Gene-Z使用一次性无阀聚合物微流控芯片，该芯片包含四个阵列，每个阵列有15个反应威尔斯，每个反应孔具有用于等温扩增的脱水引物，Gene-Z能够同时分析四个样品，每个样品平行地分析多个遗传标记，每个样品仅需要单个移液步骤进行分配。为了大幅降低定量所需的实时检测器的成本和尺寸，用高浓度的SYTO-81（一种非抑制性荧光DNA结合染料）进行环介导等温扩增（LAMP）。Gene-Z使用iPod Touch进行操作，iPod Touch还通过WiFi接口接收数据并进行自动分析和报告。本研究提供了与设备性能相关的数据，包括使用大肠杆菌和金黄色葡萄球菌基因组DNA的灵敏度和重现性。总的来说，Gene-Z代表了迈向真正廉价和紧凑的POC基因检测工具的重要一步。
By 2012, point of care (POC) testing will constitute roughly one third of the $59 billion in vitro diagnostics market. The ability to carry out multiplexed genetic testing and wireless connectivity are emerging as key attributes of future POC devices. In this study, an inexpensive, user-friendly and compact device (termed Gene-Z) is presented for rapid quantitative detection of multiple genetic markers with high sensitivity and specificity. Using a disposable valve-less polymer microfluidic chip containing four arrays of 15 reaction wells each with dehydrated primers for isothermal amplification, the Gene-Z enables simultaneous analysis of four samples, each for multiple genetic markers in parallel, requiring only a single pipetting step per sample for dispensing. To drastically reduce the cost and size of the real-time detector necessary for quantification, loop-mediated isothermal amplification (LAMP) was performed with a high concentration of SYTO-81, a non-inhibiting fluorescent DNA binding dye. The Gene-Z is operated using an iPod Touch, which also receives data and carries out automated analysis and reporting via a WiFi interface. This study presents data pertaining to performance of the device including sensitivity and reproducibility using genomic DNA from Escherichia coli and Staphylococcus aureus. Overall, the Gene-Z represents a significant step toward truly inexpensive and compact tools for POC genetic testing.