Tumor-infiltrating macrophages correlate with adverse prognosis and Epstein-Barr virus status in classical Hodgkin's lymphoma
Tumor-infiltrating macrophages correlate with adverse prognosis and Epstein-Barr virus status in classical Hodgkin's lymphoma
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DOI：
10.3324/haematol.2010.031542
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发表时间：
2011-02-01
期刊：
影响因子：
--
通讯作者：
d'Amore, Francesco
中科院分区：
文献类型：
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作者：
Kamper, Peter;Bendix, Knud;d'Amore, Francesco
背景：经典型霍奇金淋巴瘤的特征是少数肿瘤细胞被异质性背景的非肿瘤细胞（包括淋巴瘤相关巨噬细胞）包围。单核细胞/巨噬细胞谱系相关抗原CD 68和CD 163的高水平表达已被认为具有促肿瘤作用。我们的研究的目的是相关的CD 68和CD 163的表达与临床病理特征和预后的一个队列的患者与以前未经治疗的霍奇金淋巴瘤.Design and MethodsA组织芯片构建从石蜡包埋的肿瘤组织从288例经典霍奇金淋巴瘤。用免疫化学方法评估CD 68和CD 163的表达，并使用点网格计数法对肿瘤内巨噬细胞浸润的程度进行评分。从临床records.ResultsThe患者的中位年龄为37岁（范围，6-86岁）的临床数据。男女比例为1.2。在经典型霍奇金淋巴瘤（n = 288）中，在单变量水平上，高CD 68和CD 163表达与较差的总生存期（分别为P=0.002和P=0.03）和无事件生存期（分别为P=0.03和P=0.04）相关。在多变量水平上，高CD 68表达仍然显著预测总生存期（P=0.004）。此外，我们还证实了CD 68和CD 163的高表达与肿瘤细胞中EB病毒的存在有关（P=0.001和P=0.0002）。巨噬细胞/单核细胞相关抗原CD 68和CD 163的高表达与不良结局和Epstein-肿瘤细胞群中的巴尔病毒。
BackgroundClassical Hodgkin's lymphoma is characterized by a minority of neoplastic cells surrounded by a heterogeneous background population of non-neoplastic cells including lymphoma-associated macrophages. High levels of expression of both the monocyte/macrophage lineage-associated antigens CD68 and CD 163 have been suggested to have pro-tumor effects. The aim of our study was to correlate expression of CD68 and CD163 with the clinico-pathological features and prognosis of a cohort of patients with previously untreated Hodgkin's lymphoma.Design and MethodsA tissue microarray was constructed from paraffin-embedded tumor tissues from 288 cases of classical Hodgkin's lymphoma. CD68 and CD163 expression was assessed immunohistochemically and the degree of macrophage infiltration within the tumor was scored using point grid counting. Clinical data were obtained from clinical records.ResultsThe patients' median age was 37 years (range, 6-86 years). The male to female ratio was 1.2. In classical Hodgkin's lymphoma (n = 288) high CD68 and CD 163 expression correlated, at the univariate level, with poorer overall survival (P=0.002 and P=0.03, respectively) and event-free survival (P=0.03 and P=0.04, respectively). At the multivariate level, high CD68 expression remained significantly predictive of overall survival (P=0.004). In addition, we demonstrated that both high CD68 and CD163 expression were associated with the presence of Epstein-Barr virus in the neoplastic cells (P=0.001 and P=0.0002, respectively).ConclusionsIn classical Hodgkin's lymphoma, high expression of the macrophage/monocyte-related antigens CD68 and CD 163 correlates with adverse outcome and with the presence of Epstein-Barr virus in the tumor cell population.