PRNP gene polymorphism frequencies for comparing possible vulnerability to BSE in Chinese bovine population
PRNP gene polymorphism frequencies for comparing possible vulnerability to BSE in Chinese bovine population
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PRNP 基因多态性频率用于比较中国牛群体对 BSE 的易感性
DOI：
10.1080/10495398.2022.2095515
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发表时间：
2022-07-21
影响因子：
3.7
通讯作者：
Xi，Dongmei
中科院分区：
文献类型：
--
作者：
He，Xiaoming;Memon，Sameeullah;Xi，Dongmei
在众多的传染性海绵状脑病 (TSE) 中，牛海绵状脑病 (BSE) 是最著名的 TSE。它是一种潜在的克雅氏病（CJD）突变，可以通过牛传染给人类。在一些动物中，朊病毒蛋白基因 (PRNP) 被认为在 TSE 易感性或耐受性中发挥着积极作用。先前的研究发现 PRNP 基因启动子和内含子 1 区域的 indels 多态性与 BSE 易感性相关。它与推定启动子中的 23 bp 插入缺失多态性和 PRNP 基因内含子 1 中的 12 bp 插入缺失多态性相关。本研究的目的是将中甸牦牛（Bos grunniens）（YK）、中甸黄牛（Bos taurus）（YC）和中甸牛（Bos primigenius taurus × Bos grunniens）（PK）的PRNPindel多态性等位基因、基因型和单倍型频率与世界范围内报道的健康或患病牛进行比较，以评估其对BSE病毒的潜在抵抗力。疯牛病。对中国牛群与全球健康和受 BSE 影响的德国和瑞士牛进行比较，结果表明健康和受影响的牛之间存在显着差异 (p< .001)。此外，与之前对中国牛群的研究相比，发现了显着的结果。在本研究中，等位基因频率 D23 在所有分析的中国牛物种中均发现高缺失，而单倍型 D12-D23 表现出对 BSE 易感性的不太显着的倾向。
Among the numerous transmissible spongiform encephalopathies (TSEs), bovine spongiform encephalopathy (BSE) is the most well-known TSEs. It is a potential Creutzfeldt–Jakob (CJD) disease mutation that can be transferred through cattle to humans. In several animals, the prion protein gene (PRNP) is recognized to take active part in TSE vulnerability or tolerance. Previous studies have found indels polymorphism inPRNPgene promoter and intron1 region linked to BSE vulnerability. It’s linked with 23 bp indels polymorphism in putative promoter and 12 bp indel in intron 1 of thePRNPgene. The aim of this study was to compare the allele, genotype and haplotype frequencies ofPRNPindel polymorphisms in Zhongdian Yak (Bos grunniens) (YK), Zhongdian Yellow cattle (Bos taurus) (YC) and Zhongdian Yakow (Bos primigenius taurus × Bos grunniens) (PK) with worldwide reported healthy or affected BSE cattle, in order to assess their potential resistance to BSE. A comparison of Chinese bovine populations with healthy and BSE-affected German and Swiss cattle from globally was conducted, and result indicating significant difference (p< .001) between healthy and affected cattle. Additionally, as compared to prior studies with Chinese bovine population, the significant results were found. In this study, the allelic frequency D23 finding high deletion in all analyzed Chinese bovine species, and haplotype D12–D23 exhibited a less significant inclination toward susceptibility to BSE.