Cytokinesis in Trypanosoma brucei differs between bloodstream and tsetse trypomastigote forms: implications for microtubule-based morphogenesis and mutant analysis.
Cytokinesis in Trypanosoma brucei differs between bloodstream and tsetse trypomastigote forms: implications for microtubule-based morphogenesis and mutant analysis.
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DOI：
10.1111/mmi.12436
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发表时间：
2013-12
影响因子：
3.6
通讯作者：
Vaughan S
中科院分区：
文献类型：
--
作者：
Wheeler RJ;Scheumann N;Wickstead B;Gull K;Vaughan S
锥虫使用以微管为中心的机制进行细胞形态发生和胞质分裂。我们使用活细胞的扫描电子显微镜和视频显微镜，首次详细描述了早期分支真核生物布氏锥虫的细胞形态发生和胞质分裂。我们概述了胞质分裂的四个不同阶段，并表明不对称的分裂折叠将两个子细胞一分为二，在分裂前立即连接两个子细胞的细胞质桥样结构。通过检测酪氨酸化α-微管蛋白作为新的或生长的微管的标记物，以及XMAP 215（一种正末端结合蛋白）的表达作为微管正末端的标记物，我们证明了在整个细胞分裂周期中底层微管细胞骨架的空间不对称性。这导致了不同微管细胞骨架模式的遗传，并证明了微管在实现胞质分裂中的主要作用。RNA干扰技术已经导致了大量的突变体，通常具有在前循环和血流生命周期形式之间的表型变化。在这里，我们显示这两个生命周期的形式，这种寄生虫在新的鞭毛生长和胞质分裂的形态发生差异。这些发现是解释血流和前循环形式RNAi表型之间差异的重要工具，涉及细胞分裂和胞质分裂缺陷期间细胞器错误定位。
Trypanosomes use a microtubule‐focused mechanism for cell morphogenesis and cytokinesis. We used scanning electron and video microscopy of living cells to provide the first detailed description of cell morphogenesis and cytokinesis in the early‐branching eukaryote Trypanosoma brucei. We outline four distinct stages of cytokinesis and show that an asymmetric division fold bisects the two daughter cells, with a cytoplasmic bridge‐like structure connecting the two daughters immediately prior to abscission. Using detection of tyrosinated α‐tubulin as a marker for new or growing microtubules and expression of XMAP215, a plus end binding protein, as a marker for microtubule plus ends we demonstrate spatial asymmetry in the underlying microtubule cytoskeleton throughout the cell division cycle. This leads to inheritance of different microtubule cytoskeletal patterns and demonstrates the major role of microtubules in achieving cytokinesis. RNA interference techniques have led to a large set of mutants, often with variations in phenotype between procyclic and bloodstream life cycle forms. Here, we show morphogenetic differences between these two life cycle forms of this parasite during new flagellum growth and cytokinesis. These discoveries are important tools to explain differences between bloodstream and procyclic form RNAi phenotypes involving organelle mis‐positioning during cell division and cytokinesis defects.
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影响因子：
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