Inheritance and genetic mapping of a gene for seedling resistance to powdery mildew in wheat line X3986-2
Inheritance and genetic mapping of a gene for seedling resistance to powdery mildew in wheat line X3986-2
复制标题
小麦品系X3986-2幼苗抗白粉病基因的遗传及遗传图谱
DOI：
10.1007/s10681-014-1178-1
复制
发表时间：
2014-11-01
期刊：
影响因子：
1.9
通讯作者：
An, Diaoguo
中科院分区：
文献类型：
--
作者：
Ma, Pengtao;Xu, Hongxing;An, Diaoguo
鉴定和定位新的抗白粉病基因对于小麦抗病育种具有重要意义。普通小麦（Triticum aestivumL.）X3986-2对27个小麦白粉病菌菌株进行了测定。sp.tritici.为了鉴定X3986-2中的Pm基因，以X3986-2和感病品系明鲜169为亲本，构建了F2群体及其衍生的F2：3品系。分离率表明存在一个单一的显性Pm位点，暂命名为PmX 3986 -2。应用群体分离分析法筛选与PmX 3986 -2连锁的分子标记。PmX 3986 -2与5D染色体上的两个SCAR标记SCAR 112和SCAR 203以及5个简单重复序列标记CFD 40、CFD 78、CFD 81、GWM 293和WMC 443连锁，其中CFD 81和SCAR 112分别位于PmX 3986 - 2两侧0.6和1.5cM处。PmX 3986 - 2可能是Pm2、Pm 46和PmLX 66位点的一个新等位基因，它与PmX 3986 - 2紧密连锁的DNA标记可用于拓宽Pm2的遗传基础，将抗病基因快速转移到感病品种中，或用于抗病育种的基因定位。
Identification and mapping new powdery mildew resistance (Pm) genes is important for resistance breeding in wheat. Common wheat (Triticum aestivumL.) line X3986-2 was tested against 27 isolates ofBlumeria graminisf. sp.tritici. To identify thePmgene(s) in X3986-2, an F2population and its derived F2:3lines were developed from a cross between X3986-2 and susceptible line Mingxian169. Segregation ratios indicated the presence of a single dominantPmlocus, tentatively designatedPmX3986-2. Bulked segregant analysis was applied to screen for molecular markers linked toPmX3986-2. Two sequence characterized amplified region (SCAR) markers SCAR112 and SCAR203, and five simple sequence repeat markers CFD40, CFD78, CFD81, GWM293 and WMC443 on chromosome 5D were linked toPmX3986-2,with CFD81 and SCAR112 flankingPmX3986-2at 0.6 and 1.5 cM, respectively. This suggests thatPmX3986-2may be a novel allele of lociPm2,Pm46andPmLX66on chromosome arm 5DS.PmX3986-2with its tightly linked DNA markers should be useful for broadening the genetic basis ofPmand rapidly transferring the resistance gene to susceptible cultivars or for us in gene pyramiding for resistance breeding.