The development of peptide-based interfacial biomaterials for generating biological functionality on the surface of bioinert materials
The development of peptide-based interfacial biomaterials for generating biological functionality on the surface of bioinert materials
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DOI：
10.1016/j.biomaterials.2008.08.042
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发表时间：
2009-01-01
期刊：
影响因子：
14
通讯作者：
Kenan, Daniel J.
中科院分区：
文献类型：
--
作者：
Meyers, Steven R.;Khoo, Xiaojuan;Kenan, Daniel J.
传统上，植入物中使用的生物材料是根据其机械性能、化学稳定性和生物相容性选择的。然而，植入式生物医学器械的耐久性和临床有效性仍然有限，部分原因是植入物界面缺乏适当的生物相互作用以及缺乏与邻近组织的整合。在本文中，我们描述了一种稳健的基于肽的涂层技术，该技术能够通过模块化生物功能肽的非共价结合来修饰现有生物材料和医疗器械的表面。这些肽含有至少一个材料结合序列和至少一个生物活性序列，因此被称为“界面生物材料”（IFBM）。IFBM可以同时结合生物材料表面，同时在材料和生物领域之间的界面处赋予其所需的生物功能。我们展示了模型IFBM将天然聚苯乙烯（生物惰性表面）转化为可支持一系列细胞活动的生物活性表面的能力。我们进一步区分了整合素相互作用不足的简单细胞附着（在某些情况下可能对下游生物学产生不利影响）与IFBM介导的生物学适当粘附、细胞扩散和细胞存活之间的区别。此外，我们表明我们可以使用涂层技术在基底上创建荧光团和细胞的空间分辨图案，并且这些图案在培养中保留其边界。(C)2008爱思唯尔有限公司保留所有权利。
Biomaterials used in implants have traditionally been selected based on their mechanical properties, chemical stability, and biocompatibility. However, the durability and clinical efficacy of implantable biomedical devices remain limited in part due to the absence of appropriate biological interactions at the implant interface and the lack of integration into adjacent tissues. Herein, we describe a robust peptide-based coating technology capable of modifying the surface of existing biomaterials and medical devices through the non-covalent binding of modular biofunctional peptides. These peptides contain at least one material binding sequence and at least one biologically active sequence and thus are termed, "Interfacial Biomaterials" (IFBMs). IFBMs can simultaneously bind the biomaterial surface while endowing it with desired biological functionalities at the interface between the material and biological realms. We demonstrate the capabilities of model IFBMs to convert native polystyrene, a bioinert surface, into a bioactive Surface that can support a range of cell activities. We further distinguish between simple cell attachment with insufficient integrin interactions, which in some cases can adversely impact downstream biology, versus biologically appropriate adhesion, cell spreading, and cell survival mediated by IFBMs. Moreover, we show that we can use the coating technology to create spatially resolved patterns of fluorophores and cells on substrates and that these patterns retain their borders in culture. (C) 2008 Elsevier Ltd. All rights reserved.