LATS2-Ajuba complex regulates gamma-tubulin recruitment to centrosomes and spindle organization during mitosis.
LATS2-Ajuba complex regulates gamma-tubulin recruitment to centrosomes and spindle organization during mitosis.
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发表时间：
2006
期刊：
影响因子：
3.5
通讯作者：
Y. Abe;Miho Ohsugi;K. Haraguchi;J. Fujimoto;Tadashi Yamamoto
中科院分区：
文献类型：
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作者：
Y. Abe;Miho Ohsugi;K. Haraguchi;J. Fujimoto;Tadashi Yamamoto
LATS2 是果蝇肿瘤抑制基因 lats/warts 的人类同源物，编码一种有丝分裂激酶，其生理作用仍有待阐明。我们进行了酵母双杂交筛选，并鉴定了 LIM 蛋白 Ajuba，作为 LATS2 的结合伴侣。 LATS2 在整个细胞周期中定位于中心体，并在有丝分裂期间与 Ajuba 相关，有助于后者的有丝分裂磷酸化。 LATS2 或 Ajuba 的耗竭会损害有丝分裂开始时中心体的 γ-微管蛋白积累和纺锤体形成，表明 LATS2-Ajuba 复合物通过将 γ-微管蛋白募集到中心体来调节纺锤体的组织。
LATS2 is a human homolog of Drosophila tumor suppressor lats/warts, and encodes a mitotic kinase whose physiological roles remain to be elucidated. We performed yeast two-hybrid screening and identified a LIM protein Ajuba, as a binding partner of LATS2. LATS2 was localized to the centrosomes throughout the cell cycle and was associated with Ajuba during mitosis, contributing to latter's mitotic phosphorylation. Depletion of LATS2 or Ajuba impaired centrosomal accumulation of gamma-tubulin and spindle formation at the onset of mitosis, suggesting that the LATS2-Ajuba complex regulates organization of the spindle apparatus through recruitment of gamma-tubulin to the centrosome.