Layer-specific integration of locomotion and sensory information in mouse barrel cortex
Layer-specific integration of locomotion and sensory information in mouse barrel cortex
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DOI：
10.1038/s41467-019-10564-8
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发表时间：
2019-06-13
影响因子：
16.6
通讯作者：
Helmchen, Fritjof
中科院分区：
文献类型：
--
作者：
Ayaz, Asli;Stauble, Andreas;Helmchen, Fritjof
在航行过程中，啮齿动物不断地用胡须对环境进行采样。运动如何调节躯体感觉皮层的神经元活动，以及它如何与胡须触摸整合仍然不清楚。在这里，我们比较了神经元活动层2/3（L2/3）和L5的桶皮质使用钙成像小鼠在触觉虚拟现实中运行。在没有触摸的情况下，这两层在跑步和伴随的搅拌过程中增加了它们的活动。只有少数神经元受到搅拌的影响。L5神经元在跑步过程中对触壁反应短暂，而L2/3神经元表现出持续的活动。L2/3中编码触觉奔跑的神经元数量较多，在触觉过程中对奔跑速度的编码更好。各层中很少有神经元对跑步过程中触觉流的突然扰动也敏感。总之，运动显著增强了跨层的桶皮质活动，L5神经元主要报告触摸条件的变化，L2/3神经元不断将触觉刺激与跑步相结合。
During navigation, rodents continually sample the environment with their whiskers. How locomotion modulates neuronal activity in somatosensory cortex, and how it is integrated with whisker-touch remains unclear. Here, we compared neuronal activity in layer 2/3 (L2/3) and L5 of barrel cortex using calcium imaging in mice running in a tactile virtual reality. Both layers increase their activity during running and concomitant whisking, in the absence of touch. Fewer neurons are modulated by whisking alone. Whereas L5 neurons respond transiently to wall-touch during running, L2/3 neurons show sustained activity. Consistently, neurons encoding running-with-touch are more abundant in L2/3 and they encode the run-speed better during touch. Few neurons across layers were also sensitive to abrupt perturbations of tactile flow during running. In summary, locomotion significantly enhances barrel cortex activity across layers with L5 neurons mainly reporting changes in touch conditions and L2/3 neurons continually integrating tactile stimuli with running.