The hemostatic effect of deacetylated chitin membrane on peritoneal injury in rabbit model
The hemostatic effect of deacetylated chitin membrane on peritoneal injury in rabbit model
复制标题
脱乙酰甲壳素膜对兔腹膜损伤模型的止血作用
DOI：
--
复制
发表时间：
2004
期刊：
影响因子：
--
通讯作者：
M. Washio
中科院分区：
文献类型：
--
作者：
M. Fukasawa;H. Abe;T. Masaoka;H. Orita;H. Horikawa;J. Campeau;M. Washio
在这项研究中，我们确定了80%脱乙酰甲壳素（DAC-80）膜对内脏和壁层腹膜磨损后术后出血的影响。日本白色家兔进行中线剖腹术，然后进行双侧腹膜侧壁磨损（4×4 cm）或肝脏表面磨损（3×2 cm）。然后用0.2mm厚的DAC-80膜覆盖受伤的表面。术后第2天，处死家兔，通过定量50 ml腹腔灌洗液中回收的红细胞数量来确定术后出血量。DAC-80膜可减少肝表面磨损后的术后出血（DAC-80膜组：2.9±0.8 × 108个/腹腔;对照组：24.6±5.9×108个/腹腔，P<0.005）。在腹膜侧壁磨损模型中应用后，未观察到相同的止血活性。我们还测量了纤溶酶原激活剂活性（PA）和尿激酶抑制剂（派）活性的巨噬细胞从术后腹腔渗出液回收的废培养基。DAC-80膜减少了肝脏表面磨损后手术后巨噬细胞的PA分泌（用DAC-80处理：2.8±0.7;对照：3.9±0.9 mPU/ml）。DAC-80膜在腹膜侧壁磨损后对PA分泌也显示出类似的作用。DAC-80膜对术后腹腔巨噬细胞分泌派无明显影响，提示DAC-80膜可能通过调节腹腔渗出巨噬细胞的纤溶活性而发挥止血作用。
In this study, we determined the effect of 80% deacetylated chitin (DAC-80) membrane on postsurgical bleeding after visceral and parietal peritoneal abrasion. Japanese white rabbits underwent a midline laparotomy followed either by a bilateral peritoneal sidewall abrasion (4×4 cm) or an abrasion of liver surface (3×2 cm). The injured surface was then covered with a 0.2 mm thick DAC-80 membrane. On postsurgical day 2, the rabbits were sacrificed and the amounts of postsurgical bleeding was determined by quantitating the number of red blood cells recovered in 50 ml peritoneal lavage fluid. The DAC-80 membrane was found to reduce postsurgical bleeding after the abrasion of liver surface (treated with DAC-80 membrane: 2.9±0.8; control: 24.6±5.9×108 cells/peritoneal cavity, P<0.005). This same hemostatic activity was not observed after application in the peritoneal sidewall abrasion model. We also measured plasminogen activator activity (PA) and urokinase inhibitory (PAI) activity in the spent culture media of macrophages recovered from the postsurgical peritoneal exudate. The DAC-80 membrane reduced the PA secretion from postsurgical macrophages after liver surface abrasion (treated with DAC-80: 2.8±0.7; control: 3.9±0.9 mPU/ml). The DAC-80 membrane also showed similar effects on PA secretion after peritoneal sidewall abrasion. No significant effects were found in the secretion of PAI by postsurgical macrophages in both surgical models.These findings suggest that the DAC-80 membrane may have hemostatic activity through the modulation of fibrinolytic activity of peritoneal exudative macrophages.
用于纤溶酶原激活剂的高灵敏度显色微量滴定板测定，可定量区分尿激酶和组织型激活剂。
DOI：
10.1016/0006-291x(87)90463-3
发表时间：
1987
影响因子：
3.1
作者：
Karlan,BY;Clark,AS;Littlefield,BA
通讯作者：
Littlefield,BA