Spectroscopic behavior of bioconjugated quantum dots
Spectroscopic behavior of bioconjugated quantum dots
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DOI：
10.1088/0268-1242/23/7/075045
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发表时间：
2008-07-01
影响因子：
1.9
通讯作者：
Phelan, C.
中科院分区：
文献类型：
--
作者：
Chornokur, G.;Ostapenko, S.;Phelan, C.
我们报告了一个短波长，“蓝色”的光致发光（PL）光谱在CdSeTe/ZnS核/壳量子点（QD）的生物共轭与几个单克隆癌症相关抗体（AB）引起的光谱位移。扫描PL光谱进行固体基板上干燥的样品在各种温度下。观察到AB化学源对PL光谱位移的影响。使用琼脂糖凝胶电泳技术确认QD-AB缀合反应。当样品在室温以上干燥时，光谱位移强烈增加，并且该过程促进。PL光谱映射揭示了在干燥的QD-AB斑点上的PL光谱位移的分布。蓝移的机制归因于由施加到生物共轭QD的局部应力引起的QD电子能级的变化。
We report on a short-wavelength, 'blue' spectral shift of the photoluminescence (PL) spectrum in CdSeTe/ZnS core/shell quantum dots (QDs) caused by bioconjugation with several monoclonal cancer-related antibodies (ABs). Scanning PL spectroscopy was performed on samples dried on solid substrates at various temperatures. The influence of the AB chemical origin on the PL spectral shift was observed. The QD-AB conjugation reaction was confirmed using the agarose gel electrophoresis technique. The spectral shift was strongly increased and the process facilitated when the samples were dried above room temperature. The PL spectroscopic mapping revealed a profile of the PL spectral shift across the dried QD-AB spot. A mechanism of the blue shift is attributed to changes in the QD electronic energy levels caused by a local stress applied to the bioconjugated QD.