Effects of anthropogenic sound on digging behavior, metabolism, Ca(2+)/Mg(2+) ATPase activity, and metabolism-related gene expression of the bivalve Sinonovacula constricta.
Effects of anthropogenic sound on digging behavior, metabolism, Ca(2+)/Mg(2+) ATPase activity, and metabolism-related gene expression of the bivalve Sinonovacula constricta.
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人为声音对双壳类缢蛏挖掘行为、代谢、Ca2/Mg2ATP酶活性及代谢相关基因表达的影响
DOI：
10.1038/srep24266
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发表时间：
2016-04-11
影响因子：
4.6
通讯作者：
Liu G
中科院分区：
文献类型：
--
作者：
Peng C;Zhao X;Liu S;Shi W;Han Y;Guo C;Jiang J;Wan H;Shen T;Liu G
在过去的十年中，人为声音显著增加。然而，迄今为止，只有少数研究探讨了它对海洋双壳贝类的影响，其生理和分子机制知之甚少。本文研究了不同类型、频率和强度的人为噪声对<$蛤（Sinonovacula constricta）挖掘行为的影响。结果表明，声强的变化引起更深的挖掘。此外，人为噪声暴露导致的O：N比和表达的10个代谢相关基因的糖酵解，脂肪酸生物合成，色氨酸代谢，三羧酸循环（TCA循环）途径的改变。在暴露于~80 dB re 1 μPa的人为声音时，所有研究中的基因表达被诱导，在~100 dB re 1 μPa的声音下被抑制。此外，与肌肉收缩和挖掘行为直接相关的足组织Ca ~（2+）/Mg ~（2+）-ATP酶活性也受到人为声强的影响。研究结果表明，声音可能被双壳类动物感知为水粒子运动的变化，并导致随后在剃刀蛤中检测到的反应。
Anthropogenic sound has increased significantly in the past decade. However, only a few studies to date have investigated its effects on marine bivalves, with little known about the underlying physiological and molecular mechanisms. In the present study, the effects of different types, frequencies, and intensities of anthropogenic sounds on the digging behavior of razor clams (Sinonovacula constricta) were investigated. The results showed that variations in sound intensity induced deeper digging. Furthermore, anthropogenic sound exposure led to an alteration in the O:N ratios and the expression of ten metabolism-related genes from the glycolysis, fatty acid biosynthesis, tryptophan metabolism, and Tricarboxylic Acid Cycle (TCA cycle) pathways. Expression of all genes under investigation was induced upon exposure to anthropogenic sound at ~80 dB re 1 μPa and repressed at ~100 dB re 1 μPa sound. In addition, the activity of Ca2+/Mg2+-ATPase in the feet tissues, which is directly related to muscular contraction and subsequently to digging behavior, was also found to be affected by anthropogenic sound intensity. The findings suggest that sound may be perceived by bivalves as changes in the water particle motion and lead to the subsequent reactions detected in razor clams.