Antibodies against swine influenza virus neutralize the pandemic influenza virus A/H1N1
Antibodies against swine influenza virus neutralize the pandemic influenza virus A/H1N1
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DOI：
10.3892/mmr.2011.410
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发表时间：
2011-03-01
影响因子：
3.4
通讯作者：
Tsukamoto, Yasuhiro
中科院分区：
文献类型：
--
作者：
Tsukamoto, Masaya;Hiroi, Satoshi;Tsukamoto, Yasuhiro
预防流感感染的最有效方法是使用安全有效的疫苗和抗病毒材料进行预防。接种疫苗后，浆细胞在各种免疫应答后产生中和抗体，从而产生针对表达相同抗原的感染因子的保护作用。然而，在新的或未知的病原体的情况下，免疫应答的发生偶尔会延迟，从而导致相当大的发病率和死亡率。因此，抗体被认为在预防传染病方面发挥重要作用。此外，抗体用于其他目的，包括诊断和免疫治疗。2009年春季初，一种新的流感病毒株引起了北美流感的爆发，称为2009年甲型H1N1流感大流行。最初，大多数人对这种病原体的免疫力很低，导致感染在世界范围内传播，产生所谓的“流行病”。我们在此报告的“免疫球蛋白卵黄（IgY）”的产生，从鸵鸟蛋与猪流感病毒疫苗株免疫的大流行性流感病毒A/H1N1的中和抗体。用这种简单的方法，一只雌性鸵鸟产生了大量的流感病毒特异性抗体。酶联免疫吸附试验和免疫细胞化学检测结果表明，免疫鸵鸟蛋制备的IgY与甲型H1N1流感病毒和猪流感病毒具有较强的交叉反应性。此外，猪流感病毒免疫产生的鸵鸟抗体可抑制甲型H1N1流感病毒诱导的红细胞聚集活性。此外，大流行病毒感染对MDCK细胞的细胞病理学影响在与抗体的共培养物中被明显抑制，表明细胞中病毒感染性的中和。总之，我们已经成功地使用猪流感病毒抗原免疫的鸵鸟蛋大规模生产了针对2009年甲型H1N1流感病毒的中和抗体。这使得能够以成本效益高的方式生产有效的抗体，这些抗体可用于面罩和空调过滤器，以防止人口感染甲型H1N1流感病毒。
The most effective method for the prevention of influenza infection would be prophylaxis with a safe and effective vaccine and anti-viral materials. After vaccination, neutralizing antibodies are generated by plasma cells following various immune responses, thus resulting in protection against an infectious agent expressing the same antigens. However, in the case of novel or unknown pathogens, the onset of immune responses is occasionally delayed, thus resulting in considerable morbidity and mortality. Antibodies are therefore considered to play an important role in preventing infectious diseases. Furthermore, antibodies are used for additional purposes, including diagnosis and immunotherapy. In the beginning of spring 2009, an outbreak of influenza in North America was caused by a novel strain of influenza virus, designated pandemic influenza A/H1N1 2009. Initially, most people had low immunity against this pathogen, resulting in the worldwide spread of the infection to produce a so-called 'pandemic'. We herein report the generation of 'immunoglobulin yolk (IgY)' neutralizing antibodies against the pandemic influenza virus A/H1N1 from ostrich eggs immunized with a swine influenza virus vaccine strain. Using this simple method, a large amount of specific antibody against the influenza virus was produced by one female ostrich. An enzyme-linked immunosorbant assay and immunocytochemistry indicated that the IgY from the immunized ostrich eggs possessed strong cross-reactivity to the pandemic influenza virus A/H1N1 2009, as well as to the swine influenza virus. Moreover, the hemaggregation activities of the erythrocytes induced by pandemic influenza A/H1N1 virus were inhibited by the ostrich antibodies generated by swine virus immunization. In addition, the cytopathological effects on MDCK cells of infection with pandemic virus were clearly inhibited in co-cultures with the antibodies, indicating the neutralizing of viral infectivity in the cells. In conclusion, we have succeeded in the mass production of neutralizing antibodies against pandemic influenza virus A/H1N1 2009 using ostrich eggs immunized with swine influenza virus antigens. This enables the cost-effective production of effective antibodies, which could be applied to facial masks and air-conditioning filters in order to prevent populations from acquiring pandemic influenza virus A/H1N1.