Characterization and partial purification of ‘renocortins’: two polypeptides formed in renal cells causing the anti‐phospholipase‐like action of glucocorticoids
Characterization and partial purification of ‘renocortins’: two polypeptides formed in renal cells causing the anti‐phospholipase‐like action of glucocorticoids
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“肾皮质素”的表征和部分纯化：肾细胞中形成的两种多肽，引起糖皮质激素的抗磷脂酶样作用
DOI：
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发表时间：
1983
影响因子：
7.3
通讯作者：
F. Russo
中科院分区：
文献类型：
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作者：
J. Cloix;O. Colard;B. Rothhut;F. Russo
1抗炎类固醇通过抑制花生四烯酸从膜磷脂库中释放，减少培养的大鼠肾髓质间质细胞中前列腺素E2（PGE2）的合成，表现出抗磷脂酶样特性。2用地塞米松10− 6 M处理细胞后，这些细胞在上清液中释放蛋白质。3该上清液能够抑制未处理细胞中的PGE 2分泌，并抑制体外系统中的磷脂酶A2活性。4通过聚焦层析分离，我们发现两种不同的蛋白质存在，其等电点分别为5.8和8.3。5使用凝胶渗透分离，我们发现存在两种蛋白质，其表观分子量分别为15，000道尔顿和30，000道尔顿。6我们的结论是，在培养的肾细胞中，抗炎类固醇诱导合成和释放两种多肽，我们将其命名为“肾皮质素”（由肾细胞中的皮质激素诱导），引起糖皮质激素的抗磷脂酶样作用。7我们的结果与其他人的结果非常一致，但由于肾细胞不直接参与炎症过程，我们认为这种类固醇诱导的现象不仅参与炎症反应，而且具有更广泛的生理相关性。
1 Anti‐inflammatory steroids reduce prostaglandin E2 (PGE2) synthesis in rat renomedullary interstitial cells in culture by inhibiting the release of arachidonic acid from membranous phospholipid stores, exhibiting antiphospholipase‐like properties. 2 After treatment of the cells with dexamethasone 10−6M, these cells release a protein in the supernatant. 3 This supernatant is able to inhibit PGE2 secretion in untreated cells and to inhibit phospholipase A2 activity in an in vitro system. 4 Using chromatofocusing separation, we showed that two distinct proteins exist with isoelectric points of 5.8 and 8.3. 5 Using gel permeation separation, we showed that two proteins exist with apparent molecular weights of 15,000 and 30,000 daltons. 6 We conclude that, in renal cells in culture, anti‐inflammatory steroids induce the synthesis and the release of two polypeptides which we have named ‘Renocortins’ (induced by corticoids in renal cells) causing the antiphospholipase‐like action of glucocorticoids. 7 Our results are in good agreement with others, but as renal cells are not directly involved in the inflammatory process, we suggest that this steroid‐induced phenomenon is not solely involved in the inflammatory reaction but is of more general physiological relevance.