Stage and season effects on cell cycle and apoptotic activities of germ cells and Sertoli cells during spermatogenesis in the spiny dogfish (Squalus acanthias)
Stage and season effects on cell cycle and apoptotic activities of germ cells and Sertoli cells during spermatogenesis in the spiny dogfish (Squalus acanthias)
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DOI：
10.1530/rep.1.00177
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发表时间：
2005-01-01
期刊：
影响因子：
3.8
通讯作者：
McClusky, LM
中科院分区：
文献类型：
--
作者：
McClusky, LM
为了了解角鲨睾丸细胞在空间和时间上成熟的过程，我们采用了标准形态测量、增殖细胞核抗原（PCNA）和末端脱氧核苷酸转移酶介导的dUTP缺口末端标记（TUNEL）免疫组织化学方法。除了未成熟的精囊（生发区，GZ;早期减数分裂前，E-PrM），在所有后续阶段的囊肿数量和总数量的生精过程中的囊肿的季节性变化。生精周期持续约2年，并在4月/5月有丝分裂-减数分裂过渡期通过细胞凋亡导致生殖细胞克隆缺失而中断，表现为变性区（ZD）。ZD在睾丸直径上的位移速率表明，后期减数分裂前（L-PrM）世代12-13需要9-10个月才能达到成熟精子细胞阶段。此外，完成精子发生的包囊数量比2年前进入精子发生的包囊数量少约4-5倍。显着的gonocytogenesis在germinalridge是一致的ZD形成在4月/5月，但它是不存在的成熟的精原细胞和减数分裂活动时恢复了秋季。而强大的支持细胞PCNA免疫反应占主导地位的GZ囊肿细胞周期活动在整个一年中，除了在春季/夏季，精原细胞和支持细胞PCNA指数在E-PrM囊肿呈负相关。PCNA在精母细胞的免疫反应是季节性的，并依赖于减数分裂的阶段。TUNEL标记仅限于精原细胞和增加阶段依赖性的PrM区域（L-PrM =中期PrM远大于E-PrM远大于GZ），与ZD的形成，在一个季节依赖性的方式。结果表明，在角鲨的正常调节因子的影响是阶段和过程特定的。
To understand the processes involved in the spatial and temporal maturation of testicular cells in Squalus acanthias, we used standard morphometry, proliferating-cell nuclear antigen (PCNA) and terminal deoxynucleotidyl transferase-mediated dUTP nick end-labelling (TUNEL) immunohistochemistry. Except for immature spermatocysts (germinal zone, GZ; early-stage premeiotic, E-PrM), the number of cysts in all subsequent stages and the total number of cysts in the spermatogenic progression varied seasonally. The spermatogenic cycle spans about 2 years and is interrupted by germcell clone deletion via apoptosis at the mitosis-meiosis transition in April/May, manifesting as a zone of degeneration (ZD). Rate of displacement of the ZD across the testis diameter indicates that late-stage premeiotic (L-PrM) generations 12-13 require 9-10 months to reach the mature-spermatid stage. Also, the number of cysts completing spermatogenesis is approximately 4-5-fold less than the number that entered spermatogenesis proper 2 years earlier. Pronounced gonocytogenesis in the germinal ridge was coincident with ZD formation in April/May, but it was absent in the fall when mature spermatogonial and meiotic activities had resumed. Whereas strong Sertoli cell PCNA immunoreactivity dominated the GZ cyst cell-cycle activities throughout the year, except during the spring/summer months, the spermatogonial- and Sertoli-cell PCNA indices in E-PrM cysts were inversely related. PCNA immunoreactivity in spermatocytes was seasonal and dependent on the stage of meiosis. TUNEL labelling was limited to spermatogonia and increased stage-dependently in the PrM region (L-PrM = mid-stage PrM much greater than E-PrM much greater than GZ), correlating with ZD formation, in a season-dependent manner. Results imply that effects of normal regulatory factors in Squalus are stage- and process-specific.