Bioinformatic prediction of the antigenic epitopes of recombinant ferritin of Echinococcus granulosus.
Bioinformatic prediction of the antigenic epitopes of recombinant ferritin of Echinococcus granulosus.
复制标题
DOI：
10.3892/mmr.2015.4575
复制
发表时间：
2016
影响因子：
3.4
通讯作者：
Xuelei Liu;Hui Zhao;Wenyan Cao;Yumei Liu;Chuntao Zhang;Xi Lan;S. Peng;H. Wen;Jianbing Ding;Xiumin Ma
中科院分区：
文献类型：
--
作者：
Xuelei Liu;Hui Zhao;Wenyan Cao;Yumei Liu;Chuntao Zhang;Xi Lan;S. Peng;H. Wen;Jianbing Ding;Xiumin Ma
棘球蚴病是一种人畜共患的寄生虫病，主要由棘球蚴幼虫引起。主要分布于新疆、青海、甘肃和宁夏等中国牧区。本研究的目的是预测细粒棘球绦虫（Eg）的T-和B-结合表位。铁蛋白，并使用在线软件分析其二级结构。Eg. T-和B-结合表位的预测铁蛋白使用IEDB、SYFPEGII和LEPS软件进行，这些软件用于识别T-和B-细胞的共同区域。本研究的结果确定了几个潜在的抗原表位的Eg。铁蛋白，包括7个高值B细胞抗原表位氨基酸序列：8 - 16、54 - 61、70 - 75、80 - 90、103 - 109、117 - 124和167 - 173;以及4个高值T细胞抗原表位氨基酸序列：85 - 93、105 - 113、133 - 141和157 - 165。此外，鉴定了包含105 - 109个氨基酸序列的组合表位区。总之，利用生物信息学方法，本研究证实了Eg的存在。四个T细胞抗原表位、七个B细胞抗原表位和一个T和B组合表位区上的铁蛋白。这些结果为进一步研究Eg的抗原性提供了重要信息。铁蛋白和高效表位疫苗的开发。
Echinococcosis is a zoonotic parasitic disease affecting humans and other mammals, which is mainly caused Echinococcus at larval stages. It is predominantly endemic in Chinese pasture regions, including Xinjiang, Qinghai, Gansu and Ningxia. The aim of the present study was to predict the T‑ and B‑combined epitopes of Echinococcus granulosus (Eg). ferritin, and to analyze its secondary structure using online software. Prediction of the T‑ and B‑combined epitopes of Eg. ferritin was performed using IEDB, SYFPEITHI and LEPS software, which are used to identify common areas of T‑ and B‑cells. The results of the present study identified several potential antigenic epitopes of Eg. ferritin, including seven B‑cell antigen epitope amino acid sequences with high values: 8‑16, 54‑61, 70‑75, 80‑90, 103‑109, 117‑124 and 167‑173; and four T‑cell antigen epitope amino acid sequences with high values: 85‑93, 105‑113, 133‑141 and 157‑165. Furthermore, a combined epitope region comprising an 105‑109 amino acid sequence was identified. In conclusion, using bioinformatic methods, the present study confirmed the existence of Eg. ferritin on four T‑cell antigen epitopes, seven B‑cell antigen epitopes, and one T‑ and B‑combined epitope region. These findings provide significant information for further investigation of the antigenicity of Eg. ferritin and the development of highly efficient epitope vaccines.