THE EVALUATION OF MORPHOLOGICAL-CHARACTERISTICS OF HUMAN SPERMATOZOA ACCORDING TO STRICTER CRITERIA
THE EVALUATION OF MORPHOLOGICAL-CHARACTERISTICS OF HUMAN SPERMATOZOA ACCORDING TO STRICTER CRITERIA
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DOI：
10.1093/oxfordjournals.humrep.a137150
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发表时间：
1990-07-01
影响因子：
6.1
通讯作者：
VANZYL, JA
中科院分区：
文献类型：
--
作者：
MENKVELD, R;STANDER, FSH;VANZYL, JA
人类精子形态学的评价在不同实验室之间，有时甚至在实验室内部差异很大。本研究的目的是确定我们实验室开发的方法是否是一种实用、可靠和可重复的方法，并确定观察者内和观察者间的差异。“正常”精子的标准是基于上宫颈管粘液中精子的外观。采用多种方法对四名观察者对26个样本的形态学评价结果进行了统计分析。巴内特法显示了高度的相对准确度与观察员之间的误差方差为2.89和19.67之间，以及高Sperman等级相关系数为0.8675和0.6537之间（P < 0.0003）。由一名观察员进行的15次重复评价的斯皮尔曼相关系数为0.9650（P < 0.0001），而单个样品重复评价的变异系数也在可接受的范围内。基于这些结果，本文中描述的方法可以在观察者之间和观察者内部获得可比和可靠的结果。从这项研究和其他研究中可以得出结论，该方法对预测预期的IVF受精、仓鼠试验和半带试验也具有良好的预后价值。
The evaluation of the morphology of human spermatozoa varies widely between and sometimes even within laboratories. The purpose of this study was to determine whether the method that has been developed in our laboratory and which resulted in the use of stricter criteria for the evaluation of sperm morphology is a practical, reliable and repeatable method and to establish the within and between observer variations. The criteria used for a ''normal'' spermatozoon are based on the appearance of spermatozoa found in the mucus of the upper endocervical canal. The results of the morphological evaluations of 26 samples by four observers were statistically analysed by various methods. The method of Barnett showed a high degree of relative accuracy between observers with error variances of between 2.89 and 19.67 as well as high Sperman rank correlation coefficients of between 0.8675 and 0.6537 (P < 0.0003). The Spearman correlation coefficient for 15 duplicate evaluations by one observer was 0.9650 (P < 0.0001) while the coefficients of variation for repeated evaluations of single samples were also within acceptable limits. Based on these results, the method described in this article allows comparable and reliable results between and within observers to be obtained. From this and other studies it can be concluded that the method also has a good prognostic value for the prediction of expected IVF fertilization, the hamster test and hemizona assay.