SIGNET RING STOMACH-CANCER - MORPHOLOGICAL CHARACTERIZATION AND ANTIGENIC PROFILE OF A NEWLY ESTABLISHED CELL-LINE (MZ-STO-1)
SIGNET RING STOMACH-CANCER - MORPHOLOGICAL CHARACTERIZATION AND ANTIGENIC PROFILE OF A NEWLY ESTABLISHED CELL-LINE (MZ-STO-1)
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DOI：
10.1016/0277-5379(87)90265-3
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发表时间：
1987-06-01
期刊：
影响因子：
--
通讯作者：
ZUMBUSCHENFELDE, KHM
中科院分区：
文献类型：
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作者：
DIPPOLD, WG;KRON, G;ZUMBUSCHENFELDE, KHM
A human gastric signet ring cancer cell line (Mz-Sto-1) was established in tissue culture from the ascites fluid of a 54-year-old patient. The tumor cells growing in tissue culture exhibit the morphological characteristics of signet ring cells in phase contrast and transmission electron microscopy. Mz-Sto-1 cells grow as monolayer with a population doubling time of 28-36 hr during exponential growth phase and show a chromosome number between 72 and 74. In the cellular DNA of Mz-Sto-1 cells no amplification of 19 oncogenes studied is observed, c-myc included. Mz-Sto-1 cells secrete 150-250 ng CEA per 107 cells in 3 days, but no AFP. In addition Mz-Sto-1 cells and 2 already established gastric cancer cell lines MKN-28 and MKN-45 express HLA- and blood group related antigens (A, Lewis). HLA-DR antigens, which are regularly detected on normal stomach epithelium, are not found on any of the 3 cultured gastric cell lines. Mz-Sto-1 cells represent the first human gastric cancer cell characterized ultrastructurally or signet ring cells. This line will be a valuable tool to study the biology and genetics of gastric carcinoma, to test cytostatic drugs and to define new antigenic markers for stomach cancer.
