B7-H3 expression in children with asthma exacerbation
B7-H3 expression in children with asthma exacerbation
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B7-H3在哮喘急性发作患儿中的表达
DOI：
10.2500/aap.2015.36.3838
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发表时间：
2015-07-01
影响因子：
2.8
通讯作者：
Ji, Wei
中科院分区：
文献类型：
--
作者：
Chen, Zhengrong;Zhang, Guangbo;Ji, Wei
背景资料：目的：探讨B7-H3在哮喘急性发作期儿童中的表达情况。采用酶联免疫吸附试验（ELISA）技术测定B7-H3水平和细胞因子（干扰素[IFN]-γ、白细胞介素[IL]-4和IL-10）。同时，临床参数，包括实验室检查结果，用力呼气量在一秒钟（FEV1）和呼出的一氧化氮fractionalnitricoxides.Results：哮喘急性发作的儿童有显着较高的水平B7-H3比对照组（4.46 +/-1.33与3.42 +/-1.48 ng/mL; p = 0.027）。哮喘急性发作组血浆IL-4水平显著高于对照组（157.98 +/-21.57 vs 121.92 +/-24.37 pg/mL; p = 0.0001），哮喘急性发作受试者的IFN-γ水平显著降低（292.73 ± 152.47 vs 421.78 ± 145.84 pg/mL; p = 0.0107）。哮喘急性发作组中近期接受吸入性皮质类固醇（ICS）治疗者B7-H3水平显著低于未接受ICS治疗者（t = 2.706; p = 0.0136）。此外，强的松治疗后B7-H3水平显著降低。哮喘急性发作组sB7-H3水平与IFN-γ水平校正后呈负相关（r（p）=-0.605; p = 0.005）。
Background: Recent studies have shown that B7-H3, a recently identified B7 family member, plays a critical role in the development of asthma.Objective: This study is to explore the expression of B7-H3 in children with asthma exacerbation.Methods: Twenty-one Chinese children with asthma exacerbation as well as 18 nonasthmatic control Chinese children were enrolled. B7-H3 level and cytokines (interferon [IFN]-gamma, interleukin [IL]-4, and IL-10) determination were performed by enzyme-linked immunosorbent assay (ELISA) technique. Meanwhile, clinical parameters including laboratory findings, forced expiratory volume in one second (FEV1) and fractional exhaled nitric oxide were obtained.Results: Children with asthma exacerbation had significantly higher levels of B7-H3 than controls (4.46 +/- 1.33 versus 3.42 +/- 1.48 ng/mL; p = 0.027). Plasma IL-4 level was significantly higher in asthma exacerbation subjects than controls (157.98 +/- 21.57 versus 121.92 +/- 24.37 pg/mL; p = 0.0001), and IFN-gamma level was significantly lower in asthma exacerbation subjects (292.73 +/- 152.47 versus 421.78 +/- 145.84 pg/mL; p = 0.0107). Level of B7-H3 in asthma exacerbation subjects with inhaled corticosteroid (ICS) treatment recently was significantly lower than subjects without ICS treatment (t = 2.706; p = 0.0136). Additionally, levels of B7-H3 decreased remarkably after prednisone treatment. Level of sB7-H3 in asthma exacerbation subjects was inversely correlated with level of IFN-gamma (r(p) = -0.605; p = 0.005) after adjustment.Conclusion: B7-H3 may play an important role in asthma exacerbation and was a useful clinical biomarker to evaluate asthma exacerbation.