Multiple copies of Shope virus DNA are present in cells of benign and malignant non-virus-producing neoplasms
Multiple copies of Shope virus DNA are present in cells of benign and malignant non-virus-producing neoplasms
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良性和恶性非病毒产生肿瘤的细胞中存在多个 Shope 病毒 DNA 拷贝
作者：
J. G. Stevens;F. Wettstein
在初步研究Shope乳头状瘤-癌系统中肿瘤的病毒学基础的过程中，研究人员确定了家兔非病毒产生的良性和恶性肿瘤中病毒基因组存在的程度。采用从棉尾兔的多产性感染乳头瘤中纯化的镍翻译放射性病毒DNA作为探针，发现(i)乳头瘤、原发性癌和转移性癌每个二倍体细胞含有10至约100拷贝的病毒基因组，相当于DNA； （ii）病毒DNA在几乎所有肿瘤细胞中都以可检测的量存在。这些结果与病毒基因组的持续存在对于诱导和维持恶性肿瘤和良性肿瘤是必要的这一观点是一致的。
In an initial efforts to characterize the virological basis of neoplasia in the Shope papilloma-carcinoma system, the extent to which the viral genome is present in non-virus-producing benign and malignant tumors in domestic rabbits was established. Employing nick-translated radioactive viral DNA purified from productively infected papillomas on cotton tail rabbits as a probe, it was found that (i) papillomas, primary carcinomas, and metastatic carcinomas contain 10 to about 100 copies of the viral genome per diploid cell equivalent of DNA and (ii) viral DNA is present in detectable amounts in essentially all neoplastic cells. These results are consistent with the suggestion that continued presence of the viral genome is necessary for induction and maintenance of malignant as well as benign neoplasms.