Spine-type-specific recruitment of newly synthesized AMPA receptors with learning
Spine-type-specific recruitment of newly synthesized AMPA receptors with learning
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DOI：
10.1126/science.1149967
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发表时间：
2008-02-22
期刊：
影响因子：
56.9
通讯作者：
Mayford, Mark
中科院分区：
文献类型：
--
作者：
Matsuo, Naoki;Reijmers, Leon;Mayford, Mark
长期记忆的稳定需要蛋白质的重新合成.在索马中合成的蛋白质如何作用于参与特定记忆的特定突触？我们研究了新合成的AMPA型谷氨酸受体（AMPAR）的动力学，其通过使用在c-fos启动子控制下表达融合到绿色荧光蛋白（GFP-GluR 1）的GluR 1亚基的转基因小鼠来诱导学习。我们发现学习相关的招聘新合成的绿色荧光蛋白-谷氨酸受体1选择性蘑菇型棘在成年海马CA1神经元恐惧条件反射后24小时。我们的研究结果与"突触标记"模型一致，该模型允许激活的突触随后捕获新合成的受体，并且还证明了蘑菇棘与学习的关键功能区别。
The stabilization of long- term memories requires de novo protein synthesis. How can proteins, synthesized in the soma, act on specific synapses that participate in a given memory? We studied the dynamics of newly synthesized AMPA- type glutamate receptors ( AMPARs) induced with learning using transgenic mice expressing the GluR1 subunit fused to green fluorescent protein ( GFP- GluR1) under control of the c- fos promoter. We found learning- associated recruitment of newly synthesized GFP- GluR1 selectively to mushroom- type spines in adult hippocampal CA1 neurons 24 hours after fear conditioning. Our results are consistent with a "synaptic tagging" model to allow activated synapses to subsequently capture newly synthesized receptor and also demonstrate a critical functional distinction in the mushroom spines with learning.