Comparison of Endothelial Differentiation Capacities of Human and Rat Adipose-Derived Stem Cells
Comparison of Endothelial Differentiation Capacities of Human and Rat Adipose-Derived Stem Cells
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DOI：
10.1097/prs.0000000000002791
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发表时间：
2016-12-01
影响因子：
3.6
通讯作者：
Sahar, David E.
中科院分区：
文献类型：
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作者：
Orbay, Hakan;Devi, Kamaljit;Sahar, David E.
背景资料：作者比较了人和大鼠脂肪源性干细胞的内皮分化能力，以确定人脂肪源性干细胞是否可以成为血管内皮cells clinically.Methods的来源：人和大鼠脂肪源性干细胞收获，并与流式细胞仪和三系分化的特点。用内皮细胞分化培养基喂养第III代至第V代的细胞长达3周。结果：人和大鼠脂肪干细胞在分化前均为CD 90（+）、CD 44（+）和CD 31（-）。细胞成功分化为成脂、成骨和成软骨谱系。内皮细胞特异性基因的表达在人和大鼠细胞分化的第二周达到峰值。在人脂肪源性干细胞中，CD 31、血管内皮生长因子受体-1、一氧化氮合酶和血管性血友病因子基因表达的倍数变化在第2周分别为0.4 +/- 0.1、34.7 +/- 0.3、2.03 +/- 0.25和12.5 +/- 0.3;在大鼠细胞中分别为1.5 ± 1.01、21.6 ± 1.7、17.9 ± 0.6和11.2 ± 1.3。在第1、2和3周，人细胞群中CD 31（+）细胞的百分比分别为0.2、0.64和1.6，大鼠细胞群中分别为0.5、5.91和11.5。大鼠脂肪干细胞衍生的内皮细胞显示增强的发芽能力与人类cells.Conclusions：人脂肪干细胞反应不强烈EGM-2 MV内皮分化培养基比大鼠细胞。尽管如此，人类细胞有潜力成为临床来源的内皮细胞的分化条件的修改。
Background: The authors compared the endothelial differentiation capacities of human and rat adipose-derived stem cells to determine whether human adipose-derived stem cells can be a source of endothelial cells clinically.Methods: Human and rat adipose-derived stem cells were harvested and characterized with flow cytometry and trilineage differentiation. Cells from passages III through V were fed with endothelial cell differentiation medium for up to 3 weeks. Cells were harvested after 1, 2, and 3 weeks, and endothelial differentiation was evaluated with quantitative reverse-transcriptase polymerase chain reaction, flow cytometry, and angiogenic sprouting assays.Results: Both human and rat adipose-derived stem cells were CD90(+), CD44(+), and CD31(-) before differentiation. The cells were successfully differentiated into adipogenic, osteogenic, and chondrogenic lineages. Expression of endothelial cell-specific genes peaked at the second week of differentiation in both human and rat cells. The fold changes in expression of CD31, vascular endothelial growth factor receptor-1, nitric oxide synthase, and von Willebrand factor genes at week 2 were 0.4 +/- 0.1, 34.7 +/- 0.3, 2.03 +/- 0.25, and 12.5 +/- 0.3 respectively, in human adipose-derived stem cells; and 1.5 +/- 1.01, 21.6 +/- 1.7, 17.9 +/- 0.6, and 11.2 +/- 1.3, respectively, in rat cells. The percentages of CD31(+) cells were 0.2, 0.64, and 1.6 in human cell populations and 0.5, 5.91, and 11.5 in rat cell populations at weeks 1, 2, and 3, respectively. Rat adipose-derived stem cell-derived endothelial cells displayed enhanced sprouting capability compared with the human cells.Conclusions: Human adipose-derived stem cells responded less strongly to EGM-2MV endothelial differentiation medium than did the rat cells. Still, the human cells have the potential to become a clinical source of endothelial cells with modifications in the differentiation conditions.